BMCB625:DNA Replication (New components): Difference between revisions
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--[[User:Chris|Chris]] 12:47, 17 April 2007 (EDT) | --[[User:Chris|Chris]] 12:47, 17 April 2007 (EDT) | ||
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This regulation would allow for a potential mechanism in controlling the replication of origin firing. It is absolutely critical to restrict replication of the chromosome to only one round per cell cycle (Domenico, Lutzmann, Mechali, Curr. Opin. Cell Biol. (2006)v18:130-136) | |||
--[[User:Chris|Chris]] 16:17, 22 April 2007 (EDT) | |||
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Revision as of 13:17, 22 April 2007
(Homework) Questions
Jon
In regards to figure 4A, why does the helicase not knock off the 3' label?
Chris
Numerous studies have suggested that the Mcm2-7 complex does not display helicase activity in vitro, however individual subcomplexes (namely Mcm-4, -6, & -7) do display helicase activity.
Also, Kearsy and Labib (1998) propesed that MCM2-7 proteins bind to chromatin in a cell-cycle dependent manner (being tightly bound in late mitosis and G1; being removed in S- and G2-phase) (Maiorano et al, Kearsy and Labib).
Why might these features be beneficial during replication of chromosomes in eukaryotes? Hint: what is the proposed function of this entire complex in the Moyer paper.
--Chris 12:47, 17 April 2007 (EDT)
ANSWER: This regulation would allow for a potential mechanism in controlling the replication of origin firing. It is absolutely critical to restrict replication of the chromosome to only one round per cell cycle (Domenico, Lutzmann, Mechali, Curr. Opin. Cell Biol. (2006)v18:130-136)
--Chris 16:17, 22 April 2007 (EDT)
Mahta
- What do you think is the best way to determine the role of post-translational modifications in the formation of the CMG complex / activation of the helicase?
- MCM4/6/7 have helicase activity together in vitro. MCM2/3/5 may be playing more of inhibitory roles. Therefore, aren't 2 MCMs enough? (ie, you could have a trimer of MCM4 for helicase activity and a trimer of MCM2 for inhibition). What does the system gain by having so many MCMs?
Jeremy
If the purification strategy in figure 1A hadn't been so stringent, what other kinds of factors might of been isolated with cdc45?
Larry
According to current understanding, what is the most plausible mechanism for CMG component interaction and how could this be tested? Larry's Question Page
--LarryGray 11:18, 19 April 2007 (EDT)
