BMCB625:Schedule: Difference between revisions
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Revision as of 11:31, 11 April 2007
General Info
- Spring 2007
- Location: BRB 603. Wednesdays from 9:30 - 11:30 (practice session) and Thursdays from 10:30 - 12:30
- How the Class Works
Week-by-Week Schedule Summary
| Date | Presenters | Topic | Evaluator/MC/Faculty |
| April 4 | Hoatlin/Dresbeck | Org Meeting | NA |
| April 12 | Chris & Mahta | DNA Replication | JF/CP |
| April 19 | Chayne & Larry | DNA Replication (New components) | JL/CS |
| April 26 | Jeremy & Chayne | Xist (ncRNA) | LG/JF |
| May 17 | Jon (Happy Birthday) & Jeremy | Nucleosome Coding | CS/MN |
| May 24 | Mahta & Chris | Exon Jxn Complex | CP/JL |
| May 31 | Larry & Jon (Happy BD Maureen) | Helicases | MN/LG |
| June 7 | Chris | Mathematics in Biology (Final Exam) | |
| June 7 | Chayne | DNA Gyrase (Final Exam) | |
| June 13 | Mahta | ncRNA (round II) (Final Exam) | |
| June 13 | Final Exam | ||
| June 14 | pol-Y (Excision Repair) (Final Exam) | ||
| June 14 | Jon | Topo (Final Exam) |
Proposed Papers
- Enlist a faculty mentor
- send them the paper
- make sure the date works
- Enlist a class member to be the other half of the presenting team
- vote by wiki?
- Make sure there is consensus (excitement) among remaining class members about the proposed paper
- again, vote can be done on wiki
- If there is great excitement about several papers, or to correct imbalances in presentation opportunities, we can consider a different format in the last couple of weeks.
Proposed Paper for Discussion--Contact Chayne for inquiries
- Nöllmann M, Stone MD, Bryant Z, Gore J, Crisona NJ, Hong SC, Mitelheiser S, Maxwell A, Bustamante C, and Cozzarelli NR. Multiple modes of Escherichia coli DNA gyrase activity revealed by force and torque. Nat Struct Mol Biol. 2007 Apr;14(4):264-71. DOI:10.1038/nsmb1213 |
Abstract:
E. coli DNA gyrase uses the energy of ATP hydrolysis to introduce essential negative supercoils into the genome, thereby working against the mechanical stresses that accumulate in supercoiled DNA. Using a magnetic-tweezers assay, we demonstrate that small changes in force and torque can switch gyrase among three distinct modes of activity. Under low mechanical stress, gyrase introduces negative supercoils by a mechanism that depends on DNA wrapping. Elevated tension or positive torque suppresses DNA wrapping, revealing a second mode of activity that resembles the activity of topoisomerase IV. This 'distal T-segment capture' mode results in active relaxation of left-handed braids and positive supercoils. A third mode is responsible for the ATP-independent relaxation of negative supercoils. We present a branched kinetic model that quantitatively accounts for all of our single-molecule results and agrees with existing biochemical data.
Proposed Paper(s) for Jon Contact Jon for inquiries
- Petreaca RC, Chiu HC, Eckelhoefer HA, Chuang C, Xu L, and Nugent CI. Chromosome end protection plasticity revealed by Stn1p and Ten1p bypass of Cdc13p. Nat Cell Biol. 2006 Jul;8(7):748-55. DOI:10.1038/ncb1430 |
- Miller KM, Rog O, and Cooper JP. Semi-conservative DNA replication through telomeres requires Taz1. Nature. 2006 Apr 6;440(7085):824-8. DOI:10.1038/nature04638 |
- Gao H, Cervantes RB, Mandell EK, Otero JH, and Lundblad V. RPA-like proteins mediate yeast telomere function. Nat Struct Mol Biol. 2007 Mar;14(3):208-14. DOI:10.1038/nsmb1205 |
Stats
- make sure each of you has a slot as presenter 1 and 2.
