BMCB625:Topo
Gao et al
Proposed Paper(s) for Jon --Contact Jon for inquiries
Evidence that two smaller subunits of RPA bind weakly and preferentially to telomeric DNA. These telomere capping proteins have functional similarities (OB fold) with ssDNA binding proteins and may be involved in the recruitment of telomere maintenance proteins.
- Gao H, Cervantes RB, Mandell EK, Otero JH, and Lundblad V. RPA-like proteins mediate yeast telomere function. Nat Struct Mol Biol. 2007 Mar;14(3):208-14. DOI:10.1038/nsmb1205 |
(Homework) Questions
OK, I have read more. I may have mis-spoke in class so I wanted to answer Chayne's question. The G-quads inhibit tolemerase (active in stem,germ, and cancer cells). The would be a rare structure in the cell too. So the ideas is that drugs that mimic them or bind to them (stabalize the G-quartet) would inhibit tolemerase. --Jonfay 13:11, 12 June 2007 (EDT)
Mahta
Q1. Do you think there is competition between the RPA and RPA-like complex for binding telomeres?
Q2. Do you think the authors provided sufficient evidence to claim that cdc13, stn1, and ten1 form a complex that is structurally and functionally similar to the RPA complex? If no, what additional experiments do they need to carry out?
Chayne
The dependence protein binding on G-quad structure in the telomeres is not addressed in this paper. As a point of discussion, should the authors have experimented with the structural requirements of binding to the telomeres? What might be expected for proteins that may differentially stabilize or inhibit G-quad formation? How could this be addressed?
Larry
Chris
Jeremy
Q1. Propose an experiment that tests whether Stn1 and Ten1 do indeed bind DNA in vivo.
Q2. Also, what additional experiment would show that the substrates used in vitro are similar in structure to telomeres?
