BMCB625: noncoding RNA (Xist)
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(Homework) Questions
Jon
If I remember correctly we were taught (in CON663) human X inactivation is random, whereas in mouse, X inactivation occurs on the paternally derived X chromosome. Is this wrong? How do the authors simulate this "human" (random) model?
Chris
- How do Chaumeil, et al., track a "single species" of mRNA transcripts?
- How to they demonstrate the absence of transcription? What is a major drawback to this method? Do they truly demonstrate "absence of transcription?"
--Chris 22:22, 23 April 2007 (EDT)
Mahta
Q1. How do you think the Xist transcript is able to exclude RNA Pol II and other transcription components from a "defined" region? How would you go about testing your hypothesis?
Q2. In relation to Q1 - what do you think the Xist domain is comprised of?
Larry
- What approach do the authors take in order to resolve the apparent contradition of early exclusion of transcription machinery within 1-2 days and other reports showing that X-linked gene repression only begins after 2 days of differentiation?
- What possible role(s) might the jarid1c gene play that would excuse it from full inactivation?
