BME100 f2013:TEMPwu6: Difference between revisions

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==Procedure==
==Feature 1: Cancer SNP-Specific Primers==


'''Smart Phone Camera Settings'''<br>
''[Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.]''
''[Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
 
* Type of Smartphone:
'''Background on the cancer-associated mutation'''<br>
** Flash:
 
** ISO setting:
A '''nulceotide''' is ''[Instructions: your answer to question 3]''. A '''polymorphism''' is ''[Instructions: your answer to question 3]''. The polymorphism rs17879961 is found in ''[Instructions: your answer to question 4]''. The clinical significance of this polymorphism is ''[Instructions: your answer to question 4]''.
** White Balance:
** Exposure:
** Saturation:
** Contrast:


''[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]''
''[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]''

Revision as of 16:53, 20 November 2013

BME 100 Fall 2013 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
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OUR COMPANY/ BRAND

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student

[Instructions: add the name of your team's company/ product here]


LAB 6 WRITE-UP

Computer-Aided Design

TinkerCAD

[Instructions: A short summary (up to five sentences) of the TinkerCAD tool and how you used it in lab on November 20th]


Implications of Using TinkerCAD for Design

[Instructions: A short paragraph discussing just one possible way to use TinkerCAD for something practical...like redesigning the OpenPCR machine, fluorimeter, camera holder, printing out some of the smaller plastic items on demand, etc. There are lots of possibilities...pick just ONE.]



Feature 1: Cancer SNP-Specific Primers

[Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.]

Background on the cancer-associated mutation

A nulceotide is [Instructions: your answer to question 3]. A polymorphism is [Instructions: your answer to question 3]. The polymorphism rs17879961 is found in [Instructions: your answer to question 4]. The clinical significance of this polymorphism is [Instructions: your answer to question 4].

[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[Instructions: See worksheet page 6.]


Solutions Used for Calibration [Instructions: See worksheet page 6.]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Samples

[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples

[Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]


TABLE GOES HERE


Fitting a Straight Line

[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]