BME100 f2013:W1200 Group13 L4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
# Step 1 | # Step 1 Place each smaple in its respective pipette tips. That is the positive control, negative control, patient 1, and patient 2. | ||
# Step 2 | # Step 2 Place the 4 pipettes inside the OpenPCR machine. | ||
# Step 3. | # Step 3 Run the machine for 1 cycle and record results. | ||
# Step 4 Repeat step 3 untill the desired number of replications is reached. | |||
'''PCR Reaction Mix''' | '''PCR Reaction Mix''' | ||
* What is in the PCR reaction mix? | * What is in the PCR reaction mix? | ||
The PCR reaction , 50uL each. the mix contains Taq DNA polymerase, MGCl2, and dNTP's. | |||
'''DNA/ primer mix''' | '''DNA/ primer mix''' | ||
* What is in the DNA/ primer mix? | * What is in the DNA/ primer mix? | ||
Each mix contains a differant template DNA all tubes have the same forward primer and reverse primer. | |||
Revision as of 13:37, 23 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program DNA Sample Set-up
DNA Sample Set-up Procedure
The PCR reaction , 50uL each. the mix contains Taq DNA polymerase, MGCl2, and dNTP's. DNA/ primer mix
Each mix contains a differant template DNA all tubes have the same forward primer and reverse primer.
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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