BME100 f2013:W1200 Group14 L4: Difference between revisions
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
The PCR is a device used by scientists to copy DNA strands and amplify them. With the usage of heating and cooling, DNA strands are separated. With the addition of polymerases, certain segments are copied multiple times in order to observe and diagnose diseases, viruses, and bacteria.<br> | |||
[[Image:openpcr_solo.jpg]] | [[Image:openpcr_solo.jpg]] | ||
Revision as of 13:26, 23 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsDNA Sample Set-up
Materials
1. 1 Cycle at 95°C for 3 minutes PCR Reaction Mix
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) The aforementioned materials are the key components in a PCR reaction. The first step of PCR is to heat the DNA to around 95 degrees celsius for around 3 minutes, and this allows the DNA to denature and unwind into two separate strands. The temperature, which is near boiling, and the required time, are both imperative to this reaction because this is the amount of energy that is required to break the hydrogen bonds between the nucleotides of the complementary strands. Once the DNA denatures, the temperature must be lowered to 57 degrees for around 30 seconds. The lowering of temperature allows for the forward and reverse primers to bind onto each single stranded-DNA. After 30 seconds of this, the temperature is once again re-heated to 72 degrees, the ideal temperature for Taq polymerase. Taq polymerase now begins constructing complementary strands to each single template strand. This continues for around 3 minutes. This cycle should repeat itself fo (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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