BME100 f2013:W1200 Group15 L4: Difference between revisions
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| [[Image:pat1.jpg|100px|thumb|Name: Patrick McFarland<br>Protocol Planning]] | | [[Image:pat1.jpg|100px|thumb|Name: Patrick McFarland<br>Protocol Planning]] | ||
| [[Image:zac1.jpg|100px|thumb|Name: Zac Roy<br>Open PCR Machine Testing]] | | [[Image:zac1.jpg|100px|thumb|Name: Zac Roy<br>Open PCR Machine Testing]] | ||
| [[Image: | | [[Image:tolvey.jpg|100px|thumb|Name: Taylor Olvey<br>Open PCR Machine Testing]] | ||
| [[Image:greg1.jpg|100px|thumb|Name: Gregory Berghorst<br>Research and Development]] | | [[Image:greg1.jpg|100px|thumb|Name: Gregory Berghorst<br>Research and Development]] | ||
| [[Image: | | [[Image:mik.png|100px|thumb|Name: Tameem Jamal<br>Open PCR Machine Testing]] | ||
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When we unplugged the LCD Display from the Circuit Board, the machine will not be able to monitor the heat source that is being applied to the DNA samples.<br><br> | When we unplugged the LCD Display from the Circuit Board, the machine will not be able to monitor the heat source that is being applied to the DNA samples.<br><br> | ||
When we unplugged the white wire that connects the Circuit Board to the Heating | When we unplugged the white wire that connects the Circuit Board to the Heating Lid, the machine lost all power and would not heat up the DNA samples to the correct temperature.<br><br> | ||
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'''Thermal Cycler Program'''<br> | '''Thermal Cycler Program'''<br> | ||
In the | In the first step, the sample is heated to 95 degrees C for three minutes. This allows the DNA to disassociate and separate. Next, Helicase unzips the DNA, and the primer is created, ready to be copied. The machine is then cooled to 57 degrees, which at this point, the DNA tries to go back together, however the primers far outnumber the DNA, which then attaches to the DNA recreating the template DNA. Finally, the solution is heated to 72 degrees, which the polymerase is activated, and extends the DNA sequence until the template is fulfilled. | ||
'''DNA Sample Set-up'''<br> | '''DNA Sample Set-up'''<br> |
Latest revision as of 12:51, 27 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the LCD Display from the Circuit Board, the machine will not be able to monitor the heat source that is being applied to the DNA samples. When we unplugged the white wire that connects the Circuit Board to the Heating Lid, the machine lost all power and would not heat up the DNA samples to the correct temperature.
The date we first tested Open PCR was October 23, 2013. The machine is fairly simple and easy to work with. The computer program for Open PCR was fast paced and very easy to understand. Little, if no, confusion using this machine occurred. The Open PCR machine creates a new experiment fairly quickly, and has absolutely no difficulty to operate the device.
ProtocolsThermal Cycler Program DNA Sample Set-up
DNA/ primer mix
Research and DevelopmentPCR - The Underlying Technology Components of the PCR reaction and what they do. Steps of Thermal cycling Structure of DNA
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