When we unplugged the LCD Display from the Circuit Board, the machine ... Will not be able to monitor the heat source that is being applied to the DNA samples.
When we unplugged the white wire that connects the Circuit Board to (part 2), the machine ... Lost all power and would not heat up the DNA samples to the correct temperature.
Test Run
The date we first tested Open PCR was October 23, 2013. The machine is fairly simple and easy to work with. The computer program for Open PCR was fast paced and very easy to understand. Little, if no, confusion using this machine occurred. The Open PCR machine creates a new experiment fairly quickly, and has absolutely no difficulty to operate the device.
Started at 12:32pm and by 1:32pm was at cycle 17.
Protocols
Thermal Cycler Program
DNA Sample Set-up
Positive Control: PCC
Patient 1 Sample 1: P11
Patient 1 Sample 2: P12
Patient 1 Sample 3: P13
Negative Control: NCC
Patient 2 Sample 1: P21
Patient 2 Sample 2: P22
Patient 2 Sample 3: P23
DNA Sample Set-up Procedure
1. Receive 8 sample tubes from Professor, containing 50μL of PCR reaction material
2. Label Samples according to table, to avoid swapping results
3. Put appropriate DNA sample into the accordingly labeled tube. Use a new pipette tip for each test tube to avoid cross contamination
4. Place the 8 sample tubes into the Thermocycler
5. Follow Thermocycler instructions above
DNA/ primer mix
1. Sample of patient's DNA
2. Forward Primer
3. Reverse Primer
Research and Development
PCR - The Underlying Technology
(Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)