BME100 f2013:W1200 Group16 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Rene Reynolds Name: John Jakoubek Name: Courtney Willson Name: Mychal Hooser Name: Ayanna Akinyemi Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine's screen turned off so the readings were no longer available. When we unplugged the white wire that connects (part 6) to (part 2), the machine's temperature of the heating block changed from 26.1 C to -40.0 C. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample Set-up PC 1 2 3 NC A B C Patient 1: 41731 Patient 2: 78042 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? The PCR reaction mix is made up of Taq DNA polymerase, MgCl2 (magnesium chloride), and dNTP's (nucleotides). The polymerase functions to replicate the DNA strand. The magnesium chloride functions as a cofactor as the process cannot proceed without its presence. The dNTP's are the prime nucleotides as they function as building blocks to produce the synthesized DNA strands. DNA/ primer mix What is in the DNA/ primer mix? The DNA/primer mix is made up of different templates of DNA. The mix consists of the same forward primer and reverse primer. Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)