BME100 f2013:W1200 Group16 L4
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine's screen turned off so the readings were no longer available. When we unplugged the white wire that connects (part 6) to (part 2), the machine's temperature of the heating block changed from 26.1 C to -40.0 C.
(Write the date you first tested Open PCR and your experience(s) with the machine) Wednesday, October 23, 2013
ProtocolsThermal Cycler Program
Patient 1: 41731 Patient 2: 78042 DNA Sample Set-up Procedure
The PCR reaction mix is made up of Taq DNA polymerase, MgCl2 (magnesium chloride), and dNTP's (nucleotides). The polymerase functions to replicate the DNA strand. The magnesium chloride functions as a cofactor as the process cannot proceed without its presence. The dNTP's are the prime nucleotides as they function as building blocks to produce the synthesized DNA strands.
The DNA/primer mix is made up of different templates of DNA. The mix consists of the same forward primer and reverse primer.
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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