BME100 f2013:W1200 Group17 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Sonia Malek Name: Micke Cox Name: Jason Yang Name: Kevin Loung Name: Andrew Prindle Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design br> PCR (short for polymerase chain reaction) is a technique used to multiply DNA molecules. Primers (short pieces of DNA made in the laboratory)and DNA polymerase (proteins that copy a cell's DNA before it divides in two) are two components that aid the PCR process by enabling the selected DNA to be replicated effectively. Both are inserted into the DNA in the PCR machine. Through a series of processes a PCR machine heats and cools the DNA that is inserted into the machine, replicating the DNA through a chain reaction. Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Set-up DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)