BME100 f2013:W1200 Group17 L5

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Sonia Malek Name: Micke Cox Name: Andrew Prindle Name: Jason Yang Name: Kevin Luong LAB 5 WRITE-UP Background Information SYBR Green Dye The process of the lab was to use the green dye to test if the green dye and the DNA shows color when placed in the fluorimeter device. The first step was to place 80uL drop of SYBR Green Dye I solution on the first 2 clear circles in the middle of the slide. The second step was to place 80uL of sample calibration solution on top of the SYBR Green dye. The next step was to adjust the slide so that the blue light can go through the center of the drop of the SYBR Green Dye. Single-Drop Fluorimeter The fluorimeter device acts as the main part of this lab and it is very vital. The flourimeter acts as the base to where it illuminates blue light through the solution. The fluorimeter heavily depends on the smart phone to capture the solution and the light that goes through it. The flourimeter basically measures the amount of light that illuminates through the SYBR Green Dye and the DNA How the Fluorescence Technique Works [Instructions: In your own words] Procedure Smart Phone Camera Settings [Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: [Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part] Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = [Instructions: See worksheet page 6.] Solutions Used for Calibration [Instructions: See worksheet page 6.] Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Samples Image of no DNA in SYBR Green I Image of 5 microg/mL of DNA in SYBR Green I Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.] TABLE GOES HERE Fitting a Straight Line [Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]