BME100 f2013:W1200 Group18 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Carlyn Harris Role(s) Name: Tori Platt Role(s) Name: Nikhil Patel Role(s) Name: Alexandra Olson Role(s) Name: Matthew Armas Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program Heated Lid: 100°C Initial Step: 95°C for 3 minutes Next Step: 35 cycles, denature at 95°C for 30 seconds, anneal at 57°C for 30 seconds, extend at 72°C for 30 seconds Final Step: 72°C for 3 minutes Final hold: 4°C DNA Sample Set-up PCC= Positive control: Cancer DNA NCC= Negative control: Non-cancer DNA P11,P12,P13= Patient 1's DNA samples P21,P22,P23= Patient 2's DNA samples Patient 1 ID: 54174 Patient 2 ID: 43184 PCC P11 P12 P13 NCC P21 P22 P23 DNA Sample Set-up Procedure 1. Label 6 50 μL disposable pipette tips with the labels given below corresponding to each patient. 2. Label the remaining 2 50 μL pipette tips, one as the positive control cancer DNA (PCC) and one as the negative control non-cancer DNA (NCC). 3. Pipette the PCR reaction mix into each reaction tube. 4. Using a different disposable pipette tip for each sample, pipette the DNA sample primer mix into each reaction tube. 5. Place the reaction tubes in the thermal cycler. 6. Run the PCR software that follows the protocol below. PCR Reaction Mix The PCR reaction mix contains: -Tag DNA Polymerase -MgCl2 -dNTP's DNA/ primer mix The DNA primer mix contains: -Different template DNAs -Forward primer -Reverse primer Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)