BME100 f2013:W1200 Group18 L4: Difference between revisions
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| [[Image: | | [[Image:Srtgsdhgf.jpg|100px|thumb|Name: Carlyn Harris<br>Role(s):Protocol]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Tori Platt<br>Role(s):Reseach and Development]] | | [[Image:BME103student.jpg|100px|thumb|Name: Tori Platt<br>Role(s):Reseach and Development]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Nikhil Patel<br>Role(s):Initial PCR Testing]] | | [[Image:BME103student.jpg|100px|thumb|Name: Nikhil Patel<br>Role(s):Initial PCR Testing]] |
Revision as of 12:07, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine's heat sensor no longer functioned. It determines how hot the machine runs, therefore the machine did not pick up any heat when it was unplugged. Test Run Open PCR was first tested on 10/23/13 from 1:18pm to 1:44pm. During the lab, we inspected the 16-tube PCR block and the heating lid, which are both designed to retain a constant temperature across the samples that would be tested. We also explored the insides of the machine, and how its various components affected one another. Our experience with the machine was somewhat frustrating because the machine failed to complete the required amount of cycles in a reasonable amount of time. It was labeled as being slow.
ProtocolsThermal Cycler Program
DNA Sample Set-up
PCR Reaction Mix The PCR reaction mix contains: -Tag DNA Polymerase -MgCl2 -dNTP's
DNA/ primer mix The DNA primer mix contains: -Different template DNAs -Forward primer -Reverse primer
Research and DevelopmentPolymerase Chain Reaction (PCR) - The Underlying Technology Polymerase chain reaction occurs when DNA is heated and cooled at exact temperatures for exact intervals of time. However, there are five components required for the reaction to work successfully; template DNA, primers, TAQ polymerase, Magnesium Chloride (MgCl2), and dNTPs (deoxyribonucletides).
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