BME100 f2013:W1200 Group1 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Chloe Kwon Role: Research and Development Name: Nima Afzalian Role: Open PCR Machine Testing Name: Casey Weinstein Role: Open PCR Machine Testing Name: Jeff Miles Role: Protocol Planning LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) The LCD screen turned off. When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) The temperature changed from 26.7 celsius degree to -40 celsius degree. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) The Open PCR was first tested on October 10th. Protocols Thermal Cycler Program DNA Sample Set-up Tube A: Positive Control: cancer DNA template Tube B: Patient 1 ID-16946 Replicate 1 Tube C: Patient 1 ID-16946 Replicate 2 Tube D: Patient 1 ID-16946 Replicate 3 Tube E: Negative Control: non-cancer DNA template Tube F: Patient 2 ID-46296 Replicate 1 Tube G: Patient 2 ID-46296 Replicate 2 Tube G: Patient 2 ID-46296 Replicate 3 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix The PCR reaction mix will contain 8 tubes. Each tube will contain 50μL of the following: Taq DNA polymerase, MgCl2, and dNTP's DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)