BME100 f2013:W1200 Group1 L4

From OpenWetWare

Revision as of 15:22, 23 October 2013 by Jeffrey R. Miles (Talk | contribs)
Jump to: navigation, search
BME 100 Fall 2013 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: Chloe KwonRole: Research and Development
Name: Chloe Kwon
Role: Research and Development
Name: Nima AfzalianRole: Open PCR Machine Testing
Name: Nima Afzalian
Role: Open PCR Machine Testing
Name: Casey WeinsteinRole: Open PCR Machine Testing
Name: Casey Weinstein
Role: Open PCR Machine Testing
Name: Jeff MilesRole: Protocol Planning
Name: Jeff Miles
Role: Protocol Planning

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)
Image:group1PCRinside.jpg

Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) The LCD screen turned off.

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) The temperature changed from 26.7 celsius degree to -40 celsius degree.

Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)
The Open PCR was first tested on October 10th.




Protocols

Thermal Cycler Program


DNA Sample Set-up

Tube A: Positive Control: cancer DNA template Tube B: Patient 1 ID-16946 Replicate 1 Tube C: Patient 1 ID-16946 Replicate 2 Tube D: Patient 1 ID-16946 Replicate 3
Tube E: Negative Control: non-cancer DNA template Tube F: Patient 2 ID-46296 Replicate 1 Tube G: Patient 2 ID-46296 Replicate 2 Tube G: Patient 2 ID-46296 Replicate 3


DNA Sample Set-up Procedure

  1. Step 1: Fill each reaction tube with 50μL of the PCR reaction mix and label all tubes according to the chart above
  2. Step 2: Add 50μL of DNA sample mix to the corresponding reaction tube (Note: use each disposable pipette tip only once to avoid cross-contamination)
  3. Step 3: Place to reaction tubes into the thermocycler and run the above program


PCR Reaction Mix The PCR reaction mix will contain 8 tubes. Each tube will contain 50μL of the following:

  • Taq DNA polymerase
  • MgCl2
  • dNTP's


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

PCR - The Underlying Technology

(Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)





Personal tools