BME100 f2013:W1200 Group2 L4: Difference between revisions
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'''PCR Reaction Components''' | '''PCR Reaction Components''' | ||
The components of the PCR Reaction include the template DNA, primers, Taq Polymerase, Magnesium Chloride (MgCl2), and Deoxyribonucleotides (dNTP's). Each component or reagent is added to the PCR tube before running them through the PCR device and software called "Open PCR". The template DNA is the source of genetic information in which the enzyme taq polymerase copies the target sequence from. The Taq Polymerase is an enzyme that can stand high temperatures, making it a thermostable enzyme that is ideal for use in the PCR reaction process. It reads the genetic information from the DNA template, proofreads, copies, and synthesizes new nucleotides to form a new strand that will pair with the 'parent' strand. Primers are pieces or fragments of DNA | The components of the PCR Reaction include the template DNA, primers, Taq Polymerase, Magnesium Chloride (MgCl2), and Deoxyribonucleotides (dNTP's). Each component or reagent is added to the PCR tube before running them through the PCR device and software called "Open PCR". The template DNA is the source of genetic information in which the enzyme taq polymerase copies the target sequence from. The Taq Polymerase is an enzyme that can stand high temperatures, making it a thermostable enzyme that is ideal for use in the PCR reaction process. It reads the genetic information from the DNA template, proofreads, copies, and synthesizes new nucleotides to form a new strand that will pair with the 'parent' strand. Primers are pieces or fragments of DNA that attach to the a single-strand of DNA. There are two primers, the 3' primer and the 5' primer which both separately attach to an opposite end of a single strand. With out the primers, the taq polymerase cannot start adding nucleotides to the existing strand. Magnesium chloride (MgCl2) acts as the buffer of the reaction and also as a cofactor to the enzyme taq polymerase. Finally, the deoxyribonucleotides (dNTP's) are the building blocks of new strand of DNA. They are nucleotides in which the taq polymerase synthesizes new nucelotides to base pair with the existing ones of the parent strand. | ||
'''2Thermal Cycling''' | '''2Thermal Cycling''' |
Revision as of 11:10, 30 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPThe Original Design Part 3 )When we unplugged the wires, coming from the screen, from the "brains/motherboard", the machine's screen went blank.
ProtocolsThermal Cycler Program
DNA Sample Set-up Procedure Materials: Procedure: Positive control: cancer Negative control: Not cancer Step 3: Add DNA/primer mix to positive control.
PCR Reaction Mix -Taq DNA Polmerase
The primer is made up of nucleic acids that serve as a starting point for DNA synthesis.
Research and DevelopmentPCR - The Underlying Technology PCR Reaction Components The components of the PCR Reaction include the template DNA, primers, Taq Polymerase, Magnesium Chloride (MgCl2), and Deoxyribonucleotides (dNTP's). Each component or reagent is added to the PCR tube before running them through the PCR device and software called "Open PCR". The template DNA is the source of genetic information in which the enzyme taq polymerase copies the target sequence from. The Taq Polymerase is an enzyme that can stand high temperatures, making it a thermostable enzyme that is ideal for use in the PCR reaction process. It reads the genetic information from the DNA template, proofreads, copies, and synthesizes new nucleotides to form a new strand that will pair with the 'parent' strand. Primers are pieces or fragments of DNA that attach to the a single-strand of DNA. There are two primers, the 3' primer and the 5' primer which both separately attach to an opposite end of a single strand. With out the primers, the taq polymerase cannot start adding nucleotides to the existing strand. Magnesium chloride (MgCl2) acts as the buffer of the reaction and also as a cofactor to the enzyme taq polymerase. Finally, the deoxyribonucleotides (dNTP's) are the building blocks of new strand of DNA. They are nucleotides in which the taq polymerase synthesizes new nucelotides to base pair with the existing ones of the parent strand. 2Thermal Cycling Thermal cycling is the process of cycling a PCR tube holding the PCR reaction in specific variations of time and temperature. The PCR reaction is first exposed to high temperatures, it is lowered, and increases slightly in temperature. enzymes and/or reagents and is done ... Initial Step Denature Anneal Extend Final Step Final Hold 3Nucleotides and Base-pairing
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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