BME100 f2013:W1200 Group2 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Amber Bengson Role: Open PCR Machine Testing Name: Chandler Varone Role: Open PCR Machine Testing Name: Prycilla Jones Role: Protocol Planning Name: Emily Mei Role: Protocol Planning Name: Terri Bivins Role: Research and Development LAB 1 WRITE-UP The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine screen went blank. (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine read -40 degrees Celsius on the screen. (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample Set-up Positive control: cancer DNA template Tube Label: cancer ID Patient 1: 43849 Replicate 1 Tube Label: 1A ID Patient 1: 43849 Replicate 2 Tube Label: 1B ID Patient 1: 43849 Replicate 3 Tube Label: 1C Negative control: cancer DNA template Tube Label: not cancer ID Patient 2: 80853 Replicate 1 Tube Label: 2A ID Patient 2: 80853 Replicate 2 Tube Label: 2B ID Patient 2: 80853 Replicate 3 Tube Label: 2C DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? -Taq DNA Polmerase -MgCl2 -dNTP's DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology PCR Reaction Components The components of the PCR Reaction include the template DNA, primers, Taq Polymerase, Magnesium Chloride(MgCl2), and Deoxyribonucleotides (dNTP's). Each component or reagent is added to the PCR tube before running them through the PCR device and software called "Open PCR".The template DNA is the source of genetic information in which an enzyme copies from. The Taq Polymerase .... Thermal Cycling Initial Step Denature Anneal Extend Final Step Final Hold Nucleotides and Base-pairing (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)