BME100 f2013:W1200 Group2 L4
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPThe Original Design Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine screen went blank. When we unplugged the white wire that connects (part 6) to (part 2), the machine read -40 degrees Celsius on the screen.
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program
DNA Sample Set-up Procedure Materials: Procedure: Positive control: cancer Negative control: Not cancer Step 3: Add DNA/primer mix to positive control.
PCR Reaction Mix -Taq DNA Polmerase
Research and DevelopmentPCR - The Underlying Technology 1PCR Reaction Components The components of the PCR Reaction include the template DNA, primers, Taq Polymerase, Magnesium Chloride (MgCl2), and Deoxyribonucleotides (dNTP's). Each component or reagent is added to the PCR tube before running them through the PCR device and software called "Open PCR". The template DNA is the source of genetic information in which an enzyme copies from. The Taq Polymerase is a thermostable enzyme. It reads the genetic information form the DNA template and checks for mutations before copying data. 2Thermal Cycling Thermal cycling is the process of cycling a PCR tube holding the PCR reaction in specific variations of time and temperature. The PCR reaction is first exposed to high temperatures, it is lowered, and increases slightly in temperature. enzymes and/or reagents and is done ... Initial Step Denature Anneal Extend Final Step Final Hold 3Nucleotides and Base-pairing
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
|