BME100 f2013:W1200 Group3 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections The OpenPCR is a technique used to copy DNA molecules. This processs is done by dramatic tempertaure changes to isolate DNA strands from one another. To begin the machine is heated to almost boiling to denature the two strands from the original DNA. Once the two strands are seperated, the temperature decreases to allow the primers to attach and bind to complimenterary matches of DNA. This initiates the coping process and the taq polmerase then attahces (slightly higher tempertaure) to add nucleotides to the sequence to extend the strand. The process is completed with the four strands of DNA. This is the first cycle and many more occur to have billions of strands duplicated. When we unplugged part 3 from part 6, the screen on the machine turned off. When we unplugged the white wire that connects part 6 to part 2, the machine would not power on and it stopped. After letting the machine run for about an hour, it was still on the first cycle. This was done on October 23,2013. Protocols Thermal Cycler Program DNA Sample Set-up Patient 1 ID: 77842 Tube: 1A Patient 1 ID: 77842 Tube: 1B Patient 1 ID: 77842 Tube: 1C Patient 1 ID: 77842 Tube:+ Control Patient 2 ID: 26204 Tube: 2A Patient 2 ID: 26204 Tube: 2B Patient 2 ID: 26204 Tube: 2C Patient 2 ID: 26204 Tube: - Contorl DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)