BME100 f2013:W1200 Group3 L4: Difference between revisions
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
[[Image: | [[Image:PCRmachine1.jpg]] | ||
The OpenPCR is a technique used to copy DNA molecules. This processs is done by dramatic tempertaure changes to isolate DNA strands from one another. To begin the machine is heated to almost boiling to denature the two strands from the original DNA. Once the two strands are seperated, the temperature decreases to allow the primers to attach and bind to complimenterary matches of DNA. This initiates the coping process and the taq polmerase then attahces (slightly higher tempertaure) to add nucleotides to the sequence to extend the strand. The process is completed with the four strands of DNA. This is the first cycle and many more occur to have billions of strands duplicated. <br> | The OpenPCR is a technique used to copy DNA molecules. This processs is done by dramatic tempertaure changes to isolate DNA strands from one another. To begin the machine is heated to almost boiling to denature the two strands from the original DNA. Once the two strands are seperated, the temperature decreases to allow the primers to attach and bind to complimenterary matches of DNA. This initiates the coping process and the taq polmerase then attahces (slightly higher tempertaure) to add nucleotides to the sequence to extend the strand. The process is completed with the four strands of DNA. This is the first cycle and many more occur to have billions of strands duplicated. <br> |
Revision as of 12:38, 30 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 4 WRITE-UPInitial Machine TestingThe Original Design The OpenPCR is a technique used to copy DNA molecules. This processs is done by dramatic tempertaure changes to isolate DNA strands from one another. To begin the machine is heated to almost boiling to denature the two strands from the original DNA. Once the two strands are seperated, the temperature decreases to allow the primers to attach and bind to complimenterary matches of DNA. This initiates the coping process and the taq polmerase then attahces (slightly higher tempertaure) to add nucleotides to the sequence to extend the strand. The process is completed with the four strands of DNA. This is the first cycle and many more occur to have billions of strands duplicated. Experimenting With the Connections When we unplugged part 3 from part 6, the screen on the machine turned off.
After letting the machine run for about an hour, it was still on the first cycle. This was done on October 23,2013.
ProtocolsThermal Cycler Program
Step 3: Add 50μL of the DNA sample Mix to each of the correspondingly labeled reaction test tubes. *Be sure to use new pipettes each time* Step 4: Start the PCR machine and wait approximately 105 minutes while the machine goes through 35 cycles based on the set-up shown directly below.
Research and DevelopmentPCR - The Underlying Technology Extra Credit:
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