BME100 f2013:W1200 Group5 L4: Difference between revisions
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| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Dominick Cocciola<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Hany Arafa<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Wendy<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Kristina<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Estafanie? idk how to spell it<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | ||
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Revision as of 18:21, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the screen from the mother board, the screen on the machine turned off. When we unplugged the white wire that connects the mother board to the PCR block, the temperature reading on the machine became incorrect and read that it was at -40°C.
On October 23, 2013 we tested the Open PCR machine and found that it worked well. The temperatures fluctuated somewhat when it went through the heat cycles, but usually only by about .1°-.2°. There was somewhat of a lag between the readings on the machine and the readings on the computer as well. As for the timing of the cycles, the machine worked correctly and cycled through the temperatures at the correct time.
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) PCR: The Process of Thermal Cycling
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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