BME100 f2013:W1200 Group5 L4: Difference between revisions
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'''The Original Design'''<br> | '''The Original Design'''<br> | ||
<i>Image 1</i> | <i>Image 1</i><br> | ||
[[Image:Openpcr5.JPG]]<br> | [[Image:Openpcr5.JPG]]<br>This is the PCR machine that we tested. <br> | ||
The Open PCR machine is a machine that uses heat cycles to replicate specific DNA strands. DNA is placed in PCR tubes, which are placed in the PCR block. The machine, which is hooked up to a computer, is programmed to cycle through different temperatures. The heated lid is set to 100°C and the machine is initially set to 95°C for three minutes. The machine then goes through 35 cycles of 95°C for 30 seconds (which splits the DNA into two strands), 57°C for 30 seconds (which is when primers bind to the target sequences and begin to replicate the strand) and then 72°C for 30 seconds (which by the end has created double the number of DNA strands each cycle started with). It is then set at 72°C for three minutes and then a final hold temperature of 4°C. | The Open PCR machine is a machine that uses heat cycles to replicate specific DNA strands. DNA is placed in PCR tubes, which are placed in the PCR block. The machine, which is hooked up to a computer, is programmed to cycle through different temperatures. The heated lid is set to 100°C and the machine is initially set to 95°C for three minutes. The machine then goes through 35 cycles of 95°C for 30 seconds (which splits the DNA into two strands), 57°C for 30 seconds (which is when primers bind to the target sequences and begin to replicate the strand) and then 72°C for 30 seconds (which by the end has created double the number of DNA strands each cycle started with). It is then set at 72°C for three minutes and then a final hold temperature of 4°C. | ||
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'''Test Run''' | '''Test Run''' | ||
On October 23, 2013 we tested the Open PCR machine and found that it worked well. The temperatures fluctuated somewhat when it went through the heat cycles, but usually only by about .1°-.2°. There was somewhat of a lag between the readings on the machine and the readings on the computer as well. As for the timing of the cycles, the machine worked correctly and cycled through the temperatures at the correct time. <br> | On October 23, 2013 we tested the Open PCR machine and found that it worked well. The temperatures fluctuated somewhat when it went through the heat cycles, but usually only by about .1°-.2°. There was somewhat of a lag between the readings on the machine and the readings on the computer as well. As for the timing of the cycles, the machine worked correctly and cycled through the temperatures at the correct time. We started at 12:55pm and at 1:40 pm our machine had gone through 15 cycles.<br> | ||
Revision as of 19:34, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the screen from the mother board, the screen on the machine turned off. When we unplugged the white wire that connects the mother board to the PCR block, the temperature reading on the machine became incorrect and read that it was at -40°C.
On October 23, 2013 we tested the Open PCR machine and found that it worked well. The temperatures fluctuated somewhat when it went through the heat cycles, but usually only by about .1°-.2°. There was somewhat of a lag between the readings on the machine and the readings on the computer as well. As for the timing of the cycles, the machine worked correctly and cycled through the temperatures at the correct time. We started at 12:55pm and at 1:40 pm our machine had gone through 15 cycles.
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) PCR: The Process of Thermal Cycling
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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