BME100 f2013:W1200 Group7 L4: Difference between revisions
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| [[Image:BME103student.jpg|100px|thumb|Name: Carlee Farhar<br>Open PCR Machine Testing]] | | [[Image:BME103student.jpg|100px|thumb|Name: Carlee Farhar<br>Open PCR Machine Testing]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Ambar Khare<br>Open PCR Machine Testing]] | | [[Image:BME103student.jpg|100px|thumb|Name: Ambar Khare<br>Open PCR Machine Testing]] | ||
| [[ | | [[|100px|thumb|Name: Zack Silverman<br>Open PCR Machine Testing)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] |
Revision as of 21:15, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAM
LAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged the LCD display (part 3) from the circuit board (part 6), the LCD display on the machine turned off. Part 6 was the circuit board and part 3 was the LCD screen display. The circuit board is the motherboard of the PCR machine. If the wire from that is disconnected from the LCD display sceen, then the circuit board wouldn't be able to relay information to the LCD screen on what needs to be displayed. When we unplugged the white wire that connects the circuit board (part 6) to the heating plate (part 2), the temperature dropped down.
Test Run We started the test run of our PCR number 17 on Wednesday October 23rd at 12:55 p.m. After the test run, we concluded that the test run was a success and it ran exactly the way we programmed it on the software. Overall, we completed 25 of the 35 cycles which was enough to let us know if the device was working properly.
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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