BME100 f2013:W1200 Group7 L4: Difference between revisions
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'''PCR - The Underlying Technology'''<br> | '''PCR - The Underlying Technology'''<br> | ||
'''PCR Functions''' | |||
The template DNA provides the segment of the DNA that we want to be replicated in the PCR. The primers attach to sites on both DNA starnds at the opposite ends. They match the segment of DNA you want to copy. They also tell the DNA polymers where to start. The Taq Polymerase is a protein whose function is to copy a cell's DNA before it divides in two. It basically finds the primers and starts adding nucleotides to the DNA strand. The magnesium Chloride is a co-factor to the DNA polymerase. It's role is similar to that of a catalyst and the PCR can't start without it. DNA polymerase adds dNTP's to the complimentary bases of DNA. These are what DNA is made of. | |||
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Revision as of 21:45, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design Experimenting With the Connections When we unplugged the LCD display (part 3) from the circuit board (part 6), the LCD display on the machine turned off. Part 6 was the circuit board and part 3 was the LCD screen display. The circuit board is the motherboard of the PCR machine. If the wire from that is disconnected from the LCD display sceen, then the circuit board wouldn't be able to relay information to the LCD screen on what needs to be displayed. When we unplugged the white wire that connects the circuit board (part 6) to the heating plate (part 2), the temperature dropped down.
Test Run We started the test run of our PCR number 17 on Wednesday October 23rd at 12:55 p.m. After the test run, we concluded that the test run was a success and it ran exactly the way we programmed it on the software. Overall, we completed 25 of the 35 cycles which was enough to let us know if the device was working properly.
ProtocolsThermal Cycler Program
Research and DevelopmentPCR - The Underlying Technology PCR Functions
The template DNA provides the segment of the DNA that we want to be replicated in the PCR. The primers attach to sites on both DNA starnds at the opposite ends. They match the segment of DNA you want to copy. They also tell the DNA polymers where to start. The Taq Polymerase is a protein whose function is to copy a cell's DNA before it divides in two. It basically finds the primers and starts adding nucleotides to the DNA strand. The magnesium Chloride is a co-factor to the DNA polymerase. It's role is similar to that of a catalyst and the PCR can't start without it. DNA polymerase adds dNTP's to the complimentary bases of DNA. These are what DNA is made of.
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