BME100 f2013:W1200 Group7 L6
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
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LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD What is TinkerCad and How we Used it:
Benefits
Feature 1: Cancer SNP-Specific Primers[Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation What is rs17879961? Primer design
Feature 2: Consumables KitAs said before, one of the problems with the kit was the fact that it was very time consuming to run the experiment. We also noticed that the pipetting was very repetitive and it became boring as time went on. After reviewing the packaging, we realized that by fixing the packaging it would make our jobs much easier. By having the tubes in the packaging stacked, it would make it easier to access the tubes ultimately making the experiment easier. We also decided that the tubes not being relabeled made things much more difficult. Even though we could label them, we noticed that the marker would come off from time to time, making it nearly impossible to run the experiment. As a company we decided to pre label the tubes with 1A, 2A, 3A, 4A, 1B, 2B, 3B, 4B, etc. This would make it easier for the scientists to write down what is in each test tube and it would be obvious by the labels. These packaging changes will make the experiments that the scientists run much easier.
Feature 3: PCR Machine HardwareThe PCR machine will be used to target DNA replication in our system. The aspect of the PCR machine that we have decided to redesign is the software and the display errors. The external software and transmission causes communication errors and can alter the results. Placing the software as an internal component rather than an external one, should also fix the display errors as well and improve the reliability of the PCR machine. The first time we used the machine half of the replications failed and and it took over 3 hours for 12 replications when it should have been more than that. This should hopefully improve the reliability as well as the speed of receiving results.
Feature 4: Fluorimeter HardwareWhen using the fluorimeter a few issues were noted. The first was when the stand holding the sample was moved the drop of solution on top slid along the glass surface. This could be fixed by increasing the sturdiness and adding wheels so it could be transported more smoothly. Also, to insure consistent distance for the camera, a stand would be connected at the optimal distance for the camera to sit. Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.] |