BME100 f2013:W1200 Group8 L4: Difference between revisions
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When we unplugged the white wire that connects the circuit board (part 6) to heating block (part 2), the machine showed a drop in temperature because the heating element lost power. | When we unplugged the white wire that connects the circuit board (part 6) to heating block (part 2), the machine showed a drop in temperature because the heating element lost power. | ||
'''Test Run''' | '''Test Run'''<br> | ||
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The date that we first tested the Open PCR was on October 23rd, 2013. We didn't think that we would be able to open up the panel and see the inner workings of this machine. We thought it was very interesting to see how the wires are connected and also how the cooling and heating system works with the layered Aluminum plates. The process of completing the entire lab did take quite a long time, but compared to other machines ours worked to perfection. | The date that we first tested the Open PCR was on October 23rd, 2013. We didn't think that we would be able to open up the panel and see the inner workings of this machine. We thought it was very interesting to see how the wires are connected and also how the cooling and heating system works with the layered Aluminum plates. The process of completing the entire lab did take quite a long time, but compared to other machines ours worked to perfection. | ||
Revision as of 14:01, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingOpenPCR is a themocycler used to conduct heat through samples of data. This is used to replicate DNA through a series of heating and cooling in order to allow certain chemical reactions to occur.The design is made to prevent any outside reagents from affecting the concentrations of the samples; certain features such as precise temperature control and a heated lid allow there to be no interference with the data. The OpenPCR conveniently connects to your computer and stores the data from every trial.
When we unplugged the LCD screen (part 3) from the circuit board (part 6), the machine's LCD screen turned off because power was cut off. When we unplugged the white wire that connects the circuit board (part 6) to heating block (part 2), the machine showed a drop in temperature because the heating element lost power. Test Run
ProtocolsThermal Cycler Program
-Taw DNA polymerase -MgCl2 -dNTP
-A reverse Primer
Research and DevelopmentPCR - The Underlying Technology
Each step of thermal Cycling Nucleotide Base Pairing
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