BME100 f2013:W1200 Group9 L4: Difference between revisions
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'''PCR - The Underlying Technology'''<br> | '''PCR - The Underlying Technology'''<br> | ||
PCR has various components. The multiple aspects have an important role in multiplying and duplicating DNA. At the start of the process, a template DNA labels the DNA selected to copy and the RNA protein which will be created. Primers are the attaching component to the PCR reaction. Simultaneously, the primers copy the DNA and stop the polymerase from attaching to the template DNA. A Taq polymerase are high resisting heat molecules that attach to the primers to match the nucleotides and copy the DNA. Magnesium Chloride is the catalyst within this reaction which helps to tag the polymerase. The last component of a PCR reaction are deoxyribonucleotides abbreviated as (dNTP's) which pairs up with the complementary bases to replicate DNA strands. | PCR has various components. The multiple aspects have an important role in multiplying and duplicating DNA. At the start of the process, a template DNA labels the DNA selected to copy and the RNA protein which will be created. Primers are the attaching component to the PCR reaction. Simultaneously, the primers copy the DNA and stop the polymerase from attaching to the template DNA. A Taq polymerase are high resisting heat molecules that attach to the primers to match the nucleotides and copy the DNA. Magnesium Chloride is the catalyst within this reaction which helps to tag the polymerase. The last component of a PCR reaction are deoxyribonucleotides abbreviated as (dNTP's) which pairs up with the complementary bases to replicate DNA strands. <br> | ||
<br> '''Components of a PCRreaction. Magnesium Chloride is not pictured.'''<br> | |||
(Source: www.extension.org/pages/32364/the-polymerase-chain-reaction-pcr#. | <br> [[Image:PCRGroup9.png]] <br> | ||
<br>'''(Source: www.extension.org/pages/32364/the-polymerase-chain-reaction-pcr#.UnBgFvmsidk)''' <br> | |||
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images.) | (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images.) |
Revision as of 18:40, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
After unplugging part 3 from part 6 of the PCR machine, the LED screen shut off. Once we reconnected the wire, the light came on again. When we unplugged the white wire that connects (part 6) to (part 2), the temperature on the screen dropped.
The date that we first worked with Open PCR was October 22, 2013 at 9:00 am. The number of the machine our group was assigned was 17.
ProtocolsThermal Cycler Program
PCR Reaction Mix
Research and DevelopmentPCR - The Underlying Technology PCR has various components. The multiple aspects have an important role in multiplying and duplicating DNA. At the start of the process, a template DNA labels the DNA selected to copy and the RNA protein which will be created. Primers are the attaching component to the PCR reaction. Simultaneously, the primers copy the DNA and stop the polymerase from attaching to the template DNA. A Taq polymerase are high resisting heat molecules that attach to the primers to match the nucleotides and copy the DNA. Magnesium Chloride is the catalyst within this reaction which helps to tag the polymerase. The last component of a PCR reaction are deoxyribonucleotides abbreviated as (dNTP's) which pairs up with the complementary bases to replicate DNA strands.
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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