BME100 f2013:W1200 Group9 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM File:.jpg Name: Jessica Stradford Role(s): Open PCR machine testing File:.jpg Name: Rachael Hall Role(s): Open PCR machine testing File:.jpg Name: Kenna Lum Role(s): Protocol Planning File:.jpg Name: Michelle Sigona Role(s): Research & Development File:.jpg Name: Salvador Avina Role(s: Research & Development) File:T.jpg Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design The Open PCR machine is a device that is used to duplicate DNA strands. The machine uses heating and cooling techniques to separate dual helix strands of DNA and then uses primers to replicate those strands. After the PCR completes its cycles, billions of new strands of DNA are created. First, DNA samples are placed into the machine along with primers. These samples are heated up to trigger the separation of the helixes and then the primers are attached to the single strands. Then the Taq polymer attaches to the primers and replicates the second strand of the DNA as the machine cools the samples, creating new strands of DNA. This process is repeated numerous times until billions of strands are made. Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... After unplugging part 3 from part 6 of the PCR machine, the LED screen shut off. Once we reconnected the wire, the light came on again. When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample Set-up row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)