BME100 f2013:W900 Group10: Difference between revisions

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The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. And the dependent varible is the inflammatory protein (Inflammotin).
The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. And the dependent varible is the inflammatory protein (Inflammotin).


==Experimental Design==
==Independent and Dependent Variables==


'''Groups'''<br>


We will have six groups.
The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. And the dependent varible is the inflammatory protein (Inflammotin).
 
 
'''Number of subjects per group'''<br>
 
Each group will consist of 20 subjects.
 
 
(Add details about your experimental design)<br>
Subject Selection:
The 120 people we recruit for this experiment will qualify as elderly based on pre-requisite medical terminology of what it takes to be called elderly. The only restriction we will place on the random aplicants is that they do not have any problems with inflammation, so they cannot have any ailment that went met with inflammation causes negative reactions to their health and they can not have arthritis or other inflammation that is present that causes them to take anti-inflammatories due to its counter productiveness on the drug being tested. The subjects should be of varying health levels, of average height and weight, and divided equally between genders to allow the ability to see if the drug acts differently. The recruitment will be random beyond these specifications
 
 
 
We will label the groups as follows, and each group will be receiving the listed dose of lipopolysaccharide on the time schedule outlined below:
 
Group 1: 0mg LPS (control group)
 
Group 2: 2mg LPS
 
Group 3: 4mg LPS
 
Group 4: 6mg LPS
 
Group 5: 8mg LPS
 
Group 6: 10mg LPS
 
Prior to the each experiment every subject of each group will have their blood drawn and their baseline Inflammotin levels recorded using an ELISA assay. This way we can determine the average baseline levels of Inflammotin in our subjects as well as record the unique baseline Inflammotin levels of each subject in order to calculate the relative change in inflommotin levels (if any) in each subject during the experiment.
 
 
 
Dose schedule:
 
 
The experiment will be conducted over a five week time period. Subjects in each group will take their designated dose beginning of every week (Monday). Then they will go through a 12 hour fasting period. The fasting period provides a more accurate reading. Additionally, food can interfere and or skew the readings, therefore fasting period is necessary.  After the 12 hour fasting period their blood will be drawn for the inflammation reading. The reason for the 5 week time span is so that subjects won’t develop tolerance to the lipopolysaccharide, and thus skew our results. Also, if the subjects are going to be fasting prior to the blood test, they need at least a week for rest and recovery until the next dose. Moreover, during the 5 week time period the subjects are not allowed to take any other sort of anti-inflammatory pills other than lipopolysaccharide. This is because other anti-inflammatory medications can interfere with the Inflammotin reading and or defeat the purpose of lipopolysaccharide.  
 
 
 
 
 
 
 
 
 
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==Subject Selection==
==Subject Selection==

Revision as of 17:19, 10 September 2013

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LAB 1 WRITE-UP

The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. And the dependent varible is the inflammatory protein (Inflammotin).

Independent and Dependent Variables

The independent variable is the dose of an inflammation inducing agent lipopolysaccharide. And the dependent varible is the inflammatory protein (Inflammotin).

Subject Selection

Select 120 elders from a pool of 220 elders ranging from 50 years of age to 80 years of age. Medical history report of each subject in the pool of 220 to inform researchers of any medical conditions that may potentially harm the subject’s due to the drug research. All participants must inform consent and have a knowledgeable understanding of the experimental design (NOTE: If participants fall under vulnerable population inform consent must be obtained from subjects legally authorized representative). Gender preference of half male and half female subject’s. Subject’s must fall within the average height to weight ratio.


(Discuss considerations of how subjects are to be selected.) The subjects will be selected at random but they will have to pass a blind survey of qualifications.




Sources of Error and Bias

• If the subjects take any other medications such as pain killers, or other daily necessary prescription could possibly skew the test reading. The chemical properties within the mediation could affect inflammation levels.


• Genetics can definitely affect inflammation levels. Everyone is different genetically therefore their response to the doses can be different as well.


(What are some of the potential sources of error or biases that could affect your results and how would you control for them?)