BME100 f2013:W900 Group10 L4: Difference between revisions
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(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)<br> | (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)<br> | ||
[[Image: BME100_Group_10_Lab_4_OpenPCR.jpg]] | [[Image: BME100_Group_10_Lab_4_OpenPCR.jpg|700px|]] | ||
Revision as of 21:52, 29 October 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program
DNA Sample Set-up Procedure Step 1: Gather the materials necessary to conduct the lab. First extract the eight samples of the desired DNA fragments and place it into the eight different test tubes in the thermal cycler. Then gather the eight samples of 50 micro-liters of the PCR reaction mix. This mix should contain Tag DNA polymerase, MgCl2, template DNAs, Primers (forward and reverse), and dNTPs.
Research and DevelopmentPCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)
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