BME100 f2013:W900 Group10 L5

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Revision as of 18:31, 9 November 2013

BME 100 Fall 2013 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
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Contents

OUR TEAM

Name: Joslin Jose
Name: Joslin Jose
Name: Barrett Anderies
Name: Barrett Anderies
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Background Information

SYBR Green Dye

SBYR green dye is a cyanine dye used as a nucleic acid stain. It binds to DNA which absorbs of blue light and emits green light. The stain binds to the double stranded DNA and stain the single stranded DNA with lower performance. It can also stain RNA with lower performance. It is also the most sensitive stain available for detecting double stranded DNA during PCR.


Single-Drop Fluorimeter


Device used to measure parameter of fluorescence such as intensity and wavelength and the display of emission spectrum after excitation by a certain spectrum of light. It detects and measures ultraviolet radiation by determining the amount of fluorescence that it produces from a phosphor fluorometric.




[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works

A droplet is placed on the hydrophobic slide which allows the droplet to hold its spherical shape. Then the slides are exposed to two light beams from either sides which decreases the surrounding noise. The upper beam passes through a filter that passes through a sample. The lower beam is passed through an attenuator and tries to match the fluorescent power given off from the sample. Both light is detected by separate transducers and turned into an electrical signal.


[Instructions: In your own words]



Procedure

Smart Phone Camera Settings
[Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:

[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[Instructions: See worksheet page 6.]


Solutions Used for Calibration [Instructions: See worksheet page 6.]

Image:BME100group10- Data sheet.png[Add more rows as needed]Placing Samples onto the Fluorimeter
  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]

Data Analysis

Representative Images of Samples

[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples

[Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]


TABLE GOES HERE


Fitting a Straight Line

[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]



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