BME100 f2013:W900 Group13 L6: Difference between revisions
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'''TinkerCAD'''<br> | '''TinkerCAD'''<br> | ||
TinkerCAD is an 3D modeling software that allows you to easily create models of your designs to be printed on a 3D printer device. After completing tutotorials where you learn how to work on various planes, modify shapes, and scale objects one can then use TinkerCad to its full potential. In this lab, we scaled a cylinder down in order for it to be used as a label on a PCR tube. We then created a new plane to place this cylinder onto the PCR tube and then added numbers to these flattened cylinder labels (an explanation of the labels can be seen below). <br> | |||
[[Image:tinkercadallison.jpg]] | [[Image:tinkercadallison.jpg]] | ||
'''Implications of Using TinkerCAD for Design'''<br> | '''Implications of Using TinkerCAD for Design'''<br> | ||
In order to improve the PCR tubes, we decided to add red and blue labels and numbers to make each sample identifiable. When we ran the PCR experiment there was some confusion as to what were the primers and the PCR mix, we labeled the tubes with a marker. However, this problem could easily be solved if the PCR tubes had built in labels, so we made this change. There now are red and blue dots on the the tube to determine the primer vs. PCR mix and numbers for various concentrations. <br> | |||
Not only can TinkerCad be used for redesigning the fluorimeter. For the fluorimeter we want to create a device that has a stand that is attached to the box and where the fluorimeter and the camera stand are attached at a set distance. To design this, the design capability associated with TinkerCad could easily be employed, making TinkerCad an extremely useful and practical tool for engineers. | |||
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==Feature 1: Cancer SNP-Specific Primers== | ==Feature 1: Cancer SNP-Specific Primers== |
Revision as of 23:07, 25 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR COMPANY[Instructions: add the name of your team's company and/or product here]
LAB 6 WRITE-UPComputer-Aided DesignTinkerCAD Implications of Using TinkerCAD for Design Not only can TinkerCad be used for redesigning the fluorimeter. For the fluorimeter we want to create a device that has a stand that is attached to the box and where the fluorimeter and the camera stand are attached at a set distance. To design this, the design capability associated with TinkerCad could easily be employed, making TinkerCad an extremely useful and practical tool for engineers.
Feature 1: Cancer SNP-Specific Primers[Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation [Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961]
Primer design
How the primers work: [Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.]
Feature 2: Consumables KitIn our kit, the consumables will be clearly labeled to differentiate between the primers, PCR Mix, and the sample DNA. The tubes in which these materials are packaged will be color-coded and labeled to avoid any confusion that could lead to mistakes in the lab. We will maintain the current micropipettor design and will not be changing the PCR tubes themselves outside of the aforementioned labeling. We will also be making the packaging tubes have a hydrophobic surface on the inside so that the materials do not stick to the inside of the tube. These changes will help prevent human error and will also help prevent excess waste from being left over. [Instructions: IF your consumables packaging plan addresses any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]
Feature 3: PCR Machine Hardware[Instructions: Summarize how you will include the PCR machine in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] This kit will include a redesigned PCR machine that features a latch to seal the PCR lid tight, a durable and heat-proof plastic design, and a removable plate for easy transport of PCR tubes. [Instructions: IF your group has decided to redesign the PCR machine to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]
Feature 4: Fluorimeter Hardware[Instructions: Summarize how you will include the fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really REALLY awesome and easy to score.] [Instructions: IF your group has decided to redesign the fluorimeter to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.] |