BME100 f2013:W900 Group14 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Rachael Brard Role(s) Name: Andrew Cable Role(s) Name: Austin Davis Role(s) Name: Omar Eltohamy Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) PCR stands for polymerase chain reaction. The PCR machine depicted here is a biochemical technology that when used properly can amplify a selected piece of dna to make millions of copies. This machine heats dna to break it apart, then cools it so that the polymerase will attach to the dna strands and make copies. Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine's screen went black. The LCD screen did not receive power while the rest of the machine still did. When we unplugged the white wire that connects (part 6) to (part 2), the machine did not read temperature properly. It briefly fluctuating upon detaching the wire, then read -40 degrees C. Test Run Wednesday, October 23, 2013 Protocols Thermal Cycler Program DNA Sample Set-up row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)