When we unplugged (part 3) from (part 6), the machine's screen went black. The LCD screen did not receive power while the rest of the machine still did.
When we unplugged the white wire that connects (part 6) to (part 2), the machine did not read temperature properly. It briefly fluctuating upon detaching the wire, then read -40 degrees C.
Test Run
Wednesday, October 23, 2013
During our test run, we had some difficulty starting up the machine, as it had troubles starting up the process, and was constantly changing the time needed to create the first batch.
Protocols
Thermal Cycler Program
1. One cycle: 95 degrees Celsius for 3 minutes.
2. 35 cycles: Denature at 95 degrees Celsius for 30s, anneal at 57 degrees Celsius for 30s, extend at 72 degrees Celsius for 30s.
3. One cycle: 72 degrees Celsius for 3 minutes
4. Hold: 4 degrees Celsius
DNA Sample Set-up
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
DNA Sample Set-up Procedure
First, the sample of DNA to be amplified is added.
Both DNA primers are then added in succession.
DNA Taq Polymerase is then added to the vials.
Finally, loose nucleic acids are added so as to form the DNA copies
PCR Reaction Mix
The PCR reaction mix is a combination of DNA to be amplified, two DNA primers, which allow for DNA Taq Polymerase to read and copy the desired sequence, DNA Taq Polymerase, for copying and reading the desired DNA, and nucleic acids, which are used to form the new copies of DNA.
DNA/ primer mix
What is in the DNA/ primer mix?
Research and Development
PCR - The Underlying Technology
(Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)