BME100 f2013:W900 Group15 L6

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Saiswathi Javangula Name: Justin Dombrowski Name: Gage Bebak Name: Ryan Fisher Name: student Name: student [Instructions: add the name of your team's company and/or product here] LAB 6 WRITE-UP Computer-Aided Design TinkerCAD TinkerCAD is an easy tool used to create digital designs that can be 3D printed into physical objects. TinkerCAD is specifically a program that allows modeling to form or refurbish a variety of products. During the previous lab, we used TinkerCAD to improve the construction of test tubes through design techniques. During the lab, we were given PCR tubes that needed minor adjustments, which could be accurately designed through TinkerCAD. We changed the layout of the test tubes by changing the shape of the lid into rectangles, which would ultimately allow for better usage of the test tubes. This would ensure for an easier and faster method to finishing up the test tube process. Implications of Using TinkerCAD for Design TinkerCAD is an excelent tool for redesigning something like the PCR machine because it becomes very easy too start with design for the PCR machine and in TinkerCAD one can easily manipulate this design by changing the size, orientation, or completely adding something. For example one change that our group thought to make to the PCR machine was to add more hole or places for test tubes in the tray under the heating pad. Using TinkerCAD it is very easy to add more of these rows of holes into the design specs. Feature 1: Cancer SNP-Specific Primers [Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation [Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961] Primer design Forward Primer: [Instructions: write the sequence of the forward primer] Cancer-specific Reverse Primer: [Instructions: write the sequence of the forward primer] How the primers work: [Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.] Feature 2: Consumables Kit The consumables for the PCR kit which our company is creating will be tightly packed within the box that everything comes in so that it does not take up extra space and be both economically logical and will not create excessive waste. The kit will come with all necessary consumables and extras of all so that no separate purchases will be made by the consumer. It will come packaged with any liquids (including primers, etc.) on bottom so that if any leak or spill occurs there is less chance of the consumables being contaminated and as a second layer of protection, the consumables will be wrapped in plastic to reduce risk of contamination. Another goal of the way our consumables will be packaged is to have the packaging be usable to the consumer and act as a disposable tray that can hold all the pipette tips and a place in the packaging to dispose of the tips and excess liquids rather than using a trashcan or other method of disposal. In order to address the difficulty of cleaning the slide using the micropipettor which often did not completely clean the slide, we will include small disposable napkins that can be used to easily clean the slides and simply toss the napkin into the biohazard disposal bin. The edges of the slides were extremely sharp and could be very harmful if they were to cut someone so to avoid this issue the slides in our PCR testing kit will be made out of sturdy disposable plastic. in order to avoid measuring errors the micropipettors will come with a lock which will lock the dials into place to avoid bumping them and measuring the liquids wrong and wasting the primers, etc. Feature 3: PCR Machine Hardware [Instructions: Summarize how you will include the PCR machine in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Feature 4: Fluorimeter Hardware [Instructions: Summarize how you will include the fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really REALLY awesome and easy to score.] [Instructions: IF your group has decided to redesign the fluorimeter to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]