BME100 f2013:W900 Group17 L4

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BME 100 Fall 2013 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Abrar Bakhsh
Role: Protocol Planning
Name: Jeremy Becker
Role: Open PCR Machine Testing
Name: Luis Hernandez
Role: Research and Development
Name: Alison Llave
Role: Open PCR Machine Testing
Name: Naaz Maududi
Role: Research and Development

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Thermal Cycler Program


DNA Sample Set-up

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • What is in the PCR reaction mix?


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

PCR - The Underlying Technology

(Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet)
Cancerous DNA will undergo an exponential growth that is shown in the images http://universe-review.ca/I11-50-PCR.jpg http://www.nature.com/scitable/nated/content/19492/pierce_18_19_large_2.jpg

https://www.google.com/searchq=process+of+pcr&source=lnms&tbm=isch&sa=X&ei=yFRwUqGTOIKxiwKY64CADw&sqi=2&ved=0CAcQ_AUoAQ&biw=1951&bih=961#q=process+of+pcr+amplification&tbm=isch&facrc=_&imgdii=_&imgrc=DFzRQQKNLcbzVM%3A%3BpKI0UaXZHG8oRM%3Bhttp%253A%252F%252Fwww.nature.com%252Fscitable%252Fnated%252Fcontent%252F19492%252Fpierce_18_19_large_2.jpg%3Bhttp%253A%252F%252Fwww.nature.com%252Fscitable%252Ftopicpage%252Fthe-biotechnology-revolution-pcr-and-the-use-553%3B700%3B323