BME100 f2013:W900 Group5 L4

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Lincoln(Grady) Bain Role: Open PCR Machine Testing Name: Andrew Olson Name: Pedro Giorge Name: Niko Vlastos Name: Omar Alsubhi LAB 1 WRITE-UP The Original Design This image shows an Open PCR machine, which is an affordable thermocycler that can perform polymerase chain reactions that amplify certain segments of dna, by using variance in temperatures to trigger certain mechanics. The process essentially replicates a single strand of DNA into multiple copies, however it must be used with a USB port and a computer through the downloadable program OpenPCR. This is how the temperature and the number of cycles can be adjusted accordingly, and all the information is downloaded straight into the computer itself, which makes data and result collection much more efficient. Experimenting With the Connections After unplugging part 3 from part 6, we had discovered that the ,machines LCD screen was part 3 and the circuit board is part 6, and when that line was severed, the LCD screen turned off completely. Then when we decided to unplug part 2 and part 6 from each other heating was completely changed from one constant to another in degrees Celsius and essentially turned the heating component off within the open PCR machine. Part 2 is the heating device/core and part 6 is the circuit board and when they were unplugged the device when from 26.7 degrees Celsius to -40 degrees Celsius. Obviously all the connections to the circuit board hold a drastic importance to the Open PCR device. Test Run The first time using an Open PCR machine was rather lack luster do to the fact that the group allowed for the machine to run for nearly a two hour period and the device barely made its way to the 6th cycle. The device was from that point deemed slow and returned to the front desk, only to be used again shortly. ( Protocols The purpose of protocol planning Protocol planning is important for many reasons, a few of which are elementary. Critical planning is put in place as the backbone that one will build upon. In other words, it is an outline of what should be done within a study or experiment or even simple things like how to label test strips. Without protocol planning experiments would contain too many variables and it would be much easier to make a mistake. Performing a study without protocol planning is like playing soccer without the rules. i.e. Protocol planning in this analogy would be the rules. In this lab we discussed the protocol for labeling test tubes along with the protocol of how to use the open PCR equipment. Protocol planning is of the utmost importance especially in a lab or while doing a study. Thermal Cycler Program DNA Sample Set-up row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development PCR - The Underlying Technology (Add a write-up, essay-style, organized into paragrpahs with descriptive headers, based on the Q&A's from Section three of your worksheet) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to credit the sources if you borrow images.)