BME100 f2013:W900 Group8 L6

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Kendall Hickie Roles: Fluorometer Specialist, Bayesian Statistics Name: student Name: student Name: student Name: Kavin Watson NinjaMed Devices LAB 6 WRITE-UP Computer-Aided Design TinkerCAD TinkerCAD is an opensource online educational website and tool one can use to create 3 dimensional representations of ones's ideas or modifications. Any 3D image one makes in TinkerCAD can be submitted to the opensource community or downloaded for one's own use. We used TinkerCAD to make a 3D representation of a modification we wished to make to a common PCR tube as shown below. Modified tube design made in TinkerCAD. Implications of Using TinkerCAD for Design Among the multitude of ways one could use TinkerCAD, one could use TinkerCAD to design or modify consumables, like tubes for example, with specific parameters one needs. Once one has designed the proper consumable, it can be exported to a 3D printer for production. Using this method, one could have tailor made consumables on demand, rather than relying on postal delivery. Feature 1: Cancer SNP-Specific Primers [Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.] Background on the cancer-associated mutation [Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961] Primer design Forward Primer: [Instructions: write the sequence of the forward primer] Cancer-specific Reverse Primer: [Instructions: write the sequence of the forward primer] How the primers work: [Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.] Feature 2: Consumables Kit [Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.] [Instructions: IF your consumables packaging plan addresses any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Feature 3: PCR Machine Hardware [Instructions: Summarize how you will include the PCR machine in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.] [Instructions: IF your group has decided to redesign the PCR machine to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.] Feature 4: Fluorimeter Hardware [Instructions: Summarize how you will include the fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really REALLY awesome and easy to score.] The fluorometer is included in the system to allow the DNA from the PCR machine to be analyzed. This redesign deals with the major weakness of the phone holder used to prop the phone up in order to take pictures of the substance in the fluorometer. Though the device had enough room to hold the phone, it was difficult to get the phone the same height as the fluorometer. This redesign allows for the reader to adjust the height of the phone adequately without having to stack objects underneath it. Though stacking objects can be effective, it causes the holder to become unstable. Even if the holder is stacked on a couple flat surfaces, the phone still has a greater chance of tipping over, which could ruin the entire experiment. This holder allows for the user to adjust the height without the use of other objects, causing the phone to be more stable and allowing for less accident in the experiment. Another advantage would be that it allows for easy set up, without having to find the right sized object to put under the holder. This allows for an easy adjustment because the nuts hold the phone holder in place while still allowing for the easy adjustment of the phone holder. This redesign permits the use of a cell phone camera, making the fluorometer process much quicker. Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach [Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via e-mail. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]