BME100 f2013:W900 Group9 L5: Difference between revisions
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'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
''[Instructions: A short summary describing SYBR green dye]''<br> | ''[Instructions: A short summary describing SYBR green dye]''<br> | ||
The SYBR green dye has the ability to fluorescene when there is a presence of dsDNA. | The SYBR green dye has the ability to fluorescene when there is a presence of dsDNA. According to Life Technologies, SYBR dyes are one of the best high sensitivity reagents for staining DNA and RNA. The green dye binds to the DNA, causing the DNA to absorb blue light and reflect green light. | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> |
Revision as of 19:54, 6 November 2013
BME 100 Fall 2013 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] The phone was placed in the cradle at an appropriate angle to view the slide from the side. The fluorimeter was raised in front of the phone to get the right angle. A light box was placed over the set up to provide darkness to take the picture of the light flowing through the DNA and SYBR Green 1. The flourence in the sample was easily seen with higher concentrations of SYBR Green 1.
[Instructions: See worksheet page 6.]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [Instructions: See worksheet page 8. To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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