BME100 f2014:Group16 L6: Difference between revisions
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| [[Image:MaebeOllie.jpg|100px|thumb|Name: Christopher Saar<br>Protocol editer<br> ]] | | [[Image:MaebeOllie.jpg|100px|thumb|Name: Christopher Saar<br>Protocol editer<br> ]] | ||
| [[Image:royce.jpg|100px|thumb|Name: Gurpaul Sidhu<br>Role(s): Wiki editor, researcher<br>Picture Source:www. shadyrecords.com/wp-content/uploads/2013/01/royce.jpg]] | | [[Image:royce.jpg|100px|thumb|Name: Gurpaul Sidhu<br>Role(s): Wiki editor, researcher<br>Picture Source:www. shadyrecords.com/wp-content/uploads/2013/01/royce.jpg]] | ||
| [[Image: | | [[Image:Bears kanye west 1280x1024 wallpaper wallpaperswa.com 75.jpg|100px|thumb|Name: Emily Angeles Mancinas<br>Picture Source:http://wallpaperswa.com]] | ||
| [[Image:DUMBO2.jpg|100px|thumb|Name: Sheania Morgan <br> image source: http://www.fandango.com/movie-photos/disney-takes-flight-from-dumbo-to-planes-886 <br>]] | | [[Image:DUMBO2.jpg|100px|thumb|Name: Sheania Morgan <br> image source: http://www.fandango.com/movie-photos/disney-takes-flight-from-dumbo-to-planes-886 <br>]] | ||
| [[Image: | | [[Image:Basset-hound1.jpg|100px|thumb|Name: Leslie Bernardino<br>Role(s): observer, recorder<br>image source: https://bassetthounds.wordpress.com/tag/basset-hounds/ ]] | ||
| [[Image:Francis.jpg|100px|thumb|Name: Romann Arizmendi]] | | [[Image:Francis.jpg|100px|thumb|Name: Romann Arizmendi]] | ||
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|'''C PCR Results for the Class'''|| | |'''C PCR Results for the Class'''|| | ||
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| | |width=700| Date across the PCR was extensive and directed several observations. The indications towards positive results relative to a positive control, and resulted of positive results with a positive diagnosis were indicated through calculations of 87percent and 7 precent respectively. The negative relative to the negative control and negative towards a negative diagnosis was low at 30 percent to 46 percent respectively. This data is far from a 99 percent accuracy but give constraints has some data considerate of data analysis and interpretation. || | ||
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'''TinkerCAD'''<br> | '''TinkerCAD'''<br> | ||
<!-- Instructions: Write a short summary (up to five sentences) of the TinkerCAD tool and how you used it during the Computer-Aided Design lab --> | <!-- Instructions: Write a short summary (up to five sentences) of the TinkerCAD tool and how you used it during the Computer-Aided Design lab --> | ||
The TinkerCAD tool functions similarly to Solidworks but instead utilizes a community based database for shapes to help complete designs. The tool was utilized to manipulate the size and holes present with the PCR machine. | |||
'''Our Design'''<br> | '''Our Design'''<br> | ||
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|width=250|[[Image:PCRfix1.png|200px|frame|center|TINKER CAD]]||width=250 |[[Image:PCRfix2.png|200px|frame|center|TINKER CAD]]|| | |width=250|[[Image:PCRfix1.png|200px|frame|center|TINKER CAD]]||width=250 |[[Image:PCRfix2.png|200px|frame|center|TINKER CAD]]|| | ||
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|This design is an improvement of the | |This design is an improvement of the original PCR design because it is larger in volume. Therefore, it can accommodate larger numbers of samples at one time. It also has a larger area to store samples which also aids in its ability to test more efficiently. It is also equipped with a button that allows for real time manipulation of the PCR process. This button is especially useful if experimenters feel as though there is a deficiency in the machine itself.|| | ||
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<!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | ||
There were no significant issues that arose based on the packaging that was utilized in this experiment. Therefore, the liquid reagents and small plastics will be packaged in the same way that they were packaged in this study. The only difference will be the plate on which the vials are stored will be wider. This is due to the change in the PCR machine design that allows it to react more reagents at once. This is not necessarily a weakness in the current kit but instead a change that is analogous to the changes that were made when the PCR machine was improved.<br> | |||
==Feature 3: Hardware - PCR Machine & Fluorimeter== | ==Feature 3: Hardware - PCR Machine & Fluorimeter== | ||
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<!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph. --> | ||
The PCR, as pictured, will operate the same within the experimental system but will be able to hold more samples and offer better user control. The fluorimeter will be unchanged, except, the button that operates the PCR machine can be detached and used on the fluorimeter. The button would be connected directly to the camera, allowing pictures to be taken without allowing light into the apparatus. This would improve the design by giving better images of the PCR results. Furthermore, the efficiency of the PCR machine would compensate for human error by increasing the window of redoing trials. | |||
Latest revision as of 20:32, 25 November 2014
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