BME100 f2014:Group2 L1

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Carilee Farrell Name: Marshall Treleven Name: Marianne Alnimri Name: Roger Rose Name: Joel Larson LAB 1 WRITE-UP Independent and Dependent Variables The independent variable in this experiment is the amount of Lipopolysaccharide because it is the variable that is being changed by the scientists. The dependent variable is Inflammotin because it is the variable in the experiment that is affected by the change in the independent variable. Experimental Design Groups There will be six groups in the experiment, and each will be given different amounts of Lipopolysaccharide. Since it has already been determined that 10 mg of Lipopolysaccharide causes an increase in Inflammotin, 10 mg is the starting point and, working down from there, the groups will take 8 mg, 6 mg, 4 mg, 2 mg, and 0 mg. The reason we would go down by 2 mg with each group is to determine the significant difference between the dosages, and narrow down the results further from there. We also want the dosages to have equal spacing between them; this would help us obtain a measurable and exact difference between the dosages. Also, with this wide range of dosage amounts, we will be able to create a graph relating the dosage of Lipopolysaccharide and the amount of Inflammotin, thereby helping us pinpoint the minimum amount of Lipopolysaccharide needed to still produce Inflammotin. Once this experiment determines the estimated minimum amount of Lipopolysaccharide (for example between 4 mg and 2 mg), other experiments could be done to more accurately pinpoint the exact minimum amount of Lipopolysaccharide. Age The age range within each group of the test subjects will be 65 to 90 years of age. Number of subjects per group Within each group there will be 10 subjects being tested. We chose 10 subjects because it is a large enough sample to give significant data, but it is not too large as to be unattainable Subject Selection We will ask for both male and female volunteers who are willing to participate in a study involving Inflammotin levels. The volunteers will be selected from retirement communities throughout the surrounding region in order to attain randomized samples. We will then screen the volunteers for the current levels of Inflammotin. Biases in the subject selection could include only choosing males or females in stead of choosing equal amounts of both genders. Subjects included in the experiment include those who are relatively healthy, uninjured, and are within the range of ages selected. Those excluded are those that are currently sick, injured, or outside of the age range. Sources of Error and Bias A source of bias would be choosing only males or females. To prevent this bias, an equal amount of males and females will be selected. A source of error could include keeping the dosage at 10mg. To control for this source of error, the dosage vary between the groups by 2mg (i.e. 10mg, 8mg, 6mg... ect). The positive control group would be the group taking 10mg of the lipopolysaccharide, while the negative control group would be the group not taking any of the lipopolysaccharide. Another source of error could include if a subject is currently sick which would cause inflimation and skew results. To control for this factor, screening for sick test subjects will be performed and only healthy subjects will be tested. Another source of error is injury to the test subject. Bruising or other forms of bodily harm can cause inflimation and skew results. To control for this factor, screening for bruised or otherwise injured subjects will be performed and only healthy subjects will be tested.