BME100 f2014:W Group2 L4

BME 100 Fall 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Victoria Bowman Role(s) Name: Andre Dang Role(s) Name: Chandler Heaton Role(s) Name: Jordan Shinn Role(s) Name: Abigail Weiss Role(s) Name: Alena Zapata Role(s) LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's. DNA primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer. A strip of empty PCR tubes Disposable pipette tips, each used only once Cup for discarded tips Micropipettor Open PCR machine PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G2 + Positive control none G2 - Negative control none G2 1-1 Patient 1, replicate 1 13209 G2 1-2 Patient 1, replicate 2 13209 G2 1-3 Patient 1, replicate 3 13209 G2 2-1 Patient 2, replicate 1 26070 G2 2-2 Patient 2, replicate 2 26070 G2 2-3 Patient 2, replicate 3 26070 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program HEATED LID: 100°C INITIAL STEP: 95°C for 2 minutes NUMBER OF CYCLES: 35 Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds. FINAL STEP: 72°C for 2 minutes FINAL HOLD: 4°C Research and Development PCR - The Underlying Technology