BME100 f2015:Group10 1030amL6
BME 100 Fall 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
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LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System
Calculation 1 dealt with the probability that a positive final test conclusion was determined given that a positive PCR reaction occurred. This is demonstrative of the reliability of the individual PCR tests based on whether or not they corresponded appropriately to the final conclusion of if the patient had the disease or not. Given that the probability of a positive final test conclusion given a positive PCR reaction was around 77%, it shows that a single PCR test is mostly reliable in determining a final conclusion of the disease and should be used tentatively as a final deciding factor when considering a diagnosis. Calculation 2 agrees with this point, as the probability of a negative final conclusion given a negative PCR test is around 88%. The reliability of a single PCR test being able to determine a final conclusion is fairly large, but when dealing with patient lives, multiple tests should be run to ensure validity of confirming or rejecting the hypothesis that the patient has the disease. For calculation 3, the probability of the patient developing the disease based on a final PCR test conclusion is around 30%, a particularly low value for a test that is supposed to determine the presence of a disease. There is a fairly low reliability of the PCR final conclusion being able to predict the development of the disease. Also, calculation 4, which dictates the probability that a patient with a negative PCR final conclusion will not develop the disease is 79%, a much higher value, although nowhere near ideal. The final PCR conclusion tests would have to be run seven times with all positive results in order for a positive test result to mean more than 90% chance of developing the disease. The final PCR conclusion test would have to be run only two times with both negative results to have greater than a 95% probability of the disease not developing. There are, however, possible sources of error that may account for this unreliability. Due to the small volumes being dispensed, it is possible that not all of the dye or sample was conveyed to the PCR tube for reaction and may have adhered to the sides of the PCR tube instead of reacting with the primers and dye. Another possible source of error includes the shielding of the sample when taking pictures for use in ImageJ. It is possible that when repeating the picture-taking process, the black box may have been sealed differently as to let more or less light in and skew the results of the color analysis. Finally, it is possible that the camera may have been positioned at different distances from the sample when the fluorescence images were being taken and could have altered the ImageJ results in that manner as well. Intro to Computer-Aided DesignTinkerCAD The design for the phone stand is different from the phone stand provided for the lab because there is no restricted compartment. A phone can be placed of any size to capture a clear picture once placed in the box. The phone stand is also supported at two ends to prevent the phone stand from falling over and a wheel is placed at one end to adjust length of area where phone is placed. In this visual, there is also small sticker strips that were designed to be used for labeling test tubes.
Feature 1: ConsumablesThe products used for consumables will be micro-pipet tips, reagents, and PCR solution mixes. The PCR consumables kit could be improved by including special labels on the PCR solution mixes. This allows for a more careful experimental procedure. The labels and pipet tips could be heat resistant so in the event that the pipets come into contact with a high temperature, there is little to no risk of any harm and the sterilization process will be a lot easier. The pipets will be manufactured with state-of-the-art durable material which makes it very useful for multiple experimental runs. The pipet tips used can be safely and easily disposed. The overall consumables kit is fairly cheap compared to other consumable kit brands while being efficient at ensuring that the lab's experiments run smoothly and hassle free.
Feature 2: Hardware - PCR Machine & Fluorimeter |