BME100 f2015:Group14 8amL5: Difference between revisions

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<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
* Type of Smartphone:
* Type of Smartphone:
** Flash: None
** Flash:
** ISO setting:
** ISO setting:
** White Balance:  
** White Balance:  
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'''Calibration'''<br>
'''Camera set-up'''<br>
<!-- INSTRUCTIONS: In the space below, briefly describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points. -->
<!-- INSTRUCTIONS: In the space below, briefly describe how to set up your camera in front of the fluorimeter. -->


<!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. -->
<!-- INSTRUCTIONS: Type the distance between your phone cradle and the drop after the equal sign. -->
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'''Placing Samples onto the Fluorimeter'''
'''Placing Samples onto the Fluorimeter'''
# Step One: Place the slide with the smooth side down and rough side up.  By doing this you allow the samples to form a bead of liquid which is ideal for viewing.<br>
# ''[Instructions: Step one, in your OWN words]''
# Step Two: Use the micropippetor to deposit the desired amount of the SYBR Green onto the slide, in line with the light.<br>
# ''[Instructions: Step two, in your own words]''
# Step Three: Eject the pipette tip into the correct Red Solo Cup, then attach a new tip to the micropipettor.<br>
# ''[Instructions: Step three, in your own words]''
# Step Four: Deposit the desired amount of the sample onto the bead of liquid.<br>
# ''[Instructions: Step etc., in your own words]''


<br>
<br>
<!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' -->
<!-- Note: Be sure to delete the instruction text in brackets: ''[ ]'' -->


==Data Analysis==
==Data Collection and Analysis==


'''Images of High, Low, and Zero Calf Thymus DNA'''
'''Images of High, Low, and Zero Calf Thymus DNA'''
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'''PCR Results: PCR concentrations solved'''
'''PCR Results: PCR concentrations solved'''
<!-- INSTRUCTIONS: Show all values from Excel Table 5 from Section 5. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.  -->
<!-- INSTRUCTIONS: Show all values from Excel Table 5 from Section 5. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.  -->
TABLE GOES HERE




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* Our positive control PCR result was ____ μg/mL
* Our positive control PCR result was ____ μg/mL
* Our negative control PCR result was ____ μg/mL
* Our negative control PCR result was ____ μg/mL


<u>Observed results</u>
<u>Observed results</u>
<!-- INSTRUCTIONS: Replace the underscore with each patient ID. After the colon, write both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed -->
<!-- INSTRUCTIONS: Replace the underscore with each patient ID. After the colon, write both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed -->
* Patient 76291 :  
* Patient _____ :  
* Patient 64291 :
* Patient _____ :
 


<u>Conclusions</u>
<u>Conclusions</u>
<!-- INSTRUCTIONS: Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. -->
<!-- INSTRUCTIONS: Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. -->
* Patient 76291 :
* Patient _____ :
* Patient 64291 :
* Patient _____ :




Revision as of 10:07, 28 October 2015

BME 100 Fall 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

PCR Reaction Report

Fluorimeter Procedure

Smart Phone Camera Settings

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Camera set-up

  • Distance between the smart phone cradle and drop =


Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Collection and Analysis

Images of High, Low, and Zero Calf Thymus DNA


Calibrator Mean Values


TABLE GOES HERE


Calibration curves


Images of Our PCR Negative and Positive Controls


PCR Results: PCR concentrations solved

TABLE GOES HERE


PCR Results: Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL


Observed results

  • Patient _____ :
  • Patient _____ :


Conclusions

  • Patient _____ :
  • Patient _____ :



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