BME100 f2015:Group15 1030amL6: Difference between revisions
(One intermediate revision by the same user not shown) | |||
Line 56: | Line 56: | ||
[[Image:Micropipette_group15_updated_design_1030.jpg|200px|descriptionofimage]] | [[Image:Micropipette_group15_updated_design_1030.jpg|200px|descriptionofimage]] | ||
<!-- Instructions: Under the image, describe your design. Why did you choose this design? How is it different from the original OpenPCR design? --><br> | <!-- Instructions: Under the image, describe your design. Why did you choose this design? How is it different from the original OpenPCR design? --><br> | ||
The micropipette was redesigned for this project due to practicality issues with the original design. The micropipette used for the OpenPCR investigation had only two clicks during the pressing of the top button that allow the user to differentiate between the different functions. However, there is a lot of capacity to use the device too quickly or to accidentally press the lever down wrong and to consequentially get air bubbles in the micropipette tip. With the new design, there are added "bumps" along the cylinder that are felt while pressing the lever down, in addition to the two larger bumps, that prevent the lever from being pressed down too quickly. By having extra blocks, the lever cannot slip too quickly and it gets much more difficult to get air bubbles into the micropipette tip. | |||
<br> | <br> | ||
Line 71: | Line 71: | ||
<!-- Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph(s). --> | <!-- Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph(s). --> | ||
Our company will include both the Open PCR machine and the fluorimeter in our system | Our company will include both the Open PCR machine and the fluorimeter in our system. The open PCR machine will be used just as it was in the lab because we found this process easy to follow, and accurate results were obtained as well. A fluorimeter with a tripod to hold the phone, and a design to keep light out will also be included in our system. The micropipette was redesigned in order to improve the accuracy with collecting and depositing samples. | ||
Latest revision as of 23:45, 24 November 2015
BME 100 Fall 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
Company:PCR-Pros
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System
What Bayes Statistics Imply about This Diagnostic Approach
Intro to Computer-Aided DesignTinkerCAD Our Design
Feature 1: ConsumablesPCR reaction kits include "very important" consumables which are non-machine materials that are necessary for a PCR reaction and for testing for disease-associated SNP's. These consumables include PCR mix, primer solution, SYBR green solution, buffer, patient and control samples, glass slides, plastic tubes, and pipette tips. Our company's PCR reaction kit will include all of the items listed above, but two of the plastic tubes will be totally black instead of clear because these two tubes will be for the SYBR green solution. These black tubes will solve the issue of the SYBR green solution being exposed to light, because the solution is sensitive to light. This product will go along with our redesigned box used with the fluorimeter which also keeps the SYBR green solution out of the light so that it shows up better in images.
Feature 2: Hardware - PCR Machine & FluorimeterOur company will include both the Open PCR machine and the fluorimeter in our system. The open PCR machine will be used just as it was in the lab because we found this process easy to follow, and accurate results were obtained as well. A fluorimeter with a tripod to hold the phone, and a design to keep light out will also be included in our system. The micropipette was redesigned in order to improve the accuracy with collecting and depositing samples.
|