BME100 f2015:Group17 1030amL4: Difference between revisions

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=LAB 3A WRITE-UP=
=LAB 4 WRITE-UP=


==Descriptive Statistics==


==Protocol==


'''Materials'''
<!-- Record all of the materials you will need for PCR -->
* Item 1
* Item 2
* etc.




'''PCR Reaction Sample List'''<br>


<!-- Fill in ALL of the blank cells. Replace the # symbols with your group’s number. For help with tables, see Wiki editing help in the main menu -->
{| {{table}}
|-
| '''Tube Label''' || '''PCR Reaction Sample''' || '''Patient ID'''
|-
| G# + || Positive control || none
|-
| G# - || Negative control || none
|-
| G# 1-1 || Patient 1, replicate 1 ||
|-
| G# 1-2 || Patient 1, replicate 2 ||
|-
| G# 1-3 || Patient 1, replicate 3 ||
|-
| G# 2-1 || Patient 2, replicate 1 ||
|-
| G# 2-2 || Patient 2, replicate 2 ||
|-
| G# 2-3 || Patient 2, replicate 3 ||
|}


<br><br>


==Results==
'''DNA Sample Set-up Procedure'''
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. -->
# Step 1
# Step 2
# Step 3...




'''OpenPCR program'''
<!-- Include a description of the thermal cycling program below. You can use text, a screen capture, a camera snapshot of the computer screen, or a digital drawing (e.g., using shapes and text boxes in Microsoft Powerpoint) -->




<br><br>
==Analysis==




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==Summary/Discussion==
==Research and Development==
When recording data, there was a comparison between gold standards and the Spree values. When recording temperature, an oral thermometer was used as the gold standard. There was very little correlation with the Spree band and the thermometer based on the Pearson R value (0.16). The closer this value is to 0, the less correlation there is between the data sets. When the Spree headband was compared to the oxidizer, our gold standard for measuring heart rate, the Pearson R value was (.823). This value shows that the gold standard and Spree values correlate well, though not perfectly. 100% correlation would involve a Pearson R value of 1. In conclusion, the Spree tracked heart rate more accurately than body temperature.
After using this headband, there were some obvious design flaws that should be addressed. First, the headband is not aesthetically pleasing; the band is bulky and has unnecessary geometric patterns covering it. After wearing the band for over an hour, it began to feel hot and uncomfortable which lead to headaches. Also, there is an unappealing red mark left on the forehead after prolonged use. In accordance with the data, the temperature recording device on the Spree is not accurate. Overall, this device is very overpriced when compared to cheaper devices that have many of the same capabilities if not more. To help combat most of these design flaws, changing the device from a headband to a chest or wrist strap would prevent unappealing looks as well as an uncomfortable feeling on the head. To provide more accurate temperature readings, it would be ideal to implement a thermometer which gives actual numbers instead of colors. As for the issue of pricing, either Spree needs to price more competitively, or the company should include more features that other cheaper devices do not include such as in depth summary/analysis of workouts and more accurate vital readings.


'''PCR - The Underlying Technology'''<br>
<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the questions and answers from the Research and Development exercise. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. You are not allowed to use images from current or past BME 100 students' reports on OpenWetWare. -->




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=LAB 3B WRITE-UP=


==Target Population and Need==


==SNP Information & Primer Design==


'''Background: About the Disease SNP'''
<!-- INSTRUCTIONS: This content is from PCR Lab B. Write a summary, at least five sentences long, about the disease SNP in your own words. -->


<br><br>


==Device Design==
'''Primer Design and Testing'''
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. -->








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==Inferential Statistics==


 
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==Graph==
 
 
 
 
 
 
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Revision as of 08:37, 14 October 2015

BME 100 Fall 2015 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: student
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Name: student
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Name: student
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LAB 4 WRITE-UP

Protocol

Materials

  • Item 1
  • Item 2
  • etc.


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G# + Positive control none
G# - Negative control none
G# 1-1 Patient 1, replicate 1
G# 1-2 Patient 1, replicate 2
G# 1-3 Patient 1, replicate 3
G# 2-1 Patient 2, replicate 1
G# 2-2 Patient 2, replicate 2
G# 2-3 Patient 2, replicate 3


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program






Research and Development

PCR - The Underlying Technology






SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing




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