BME100 f2015:Group1 1030amL6: Difference between revisions
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==Feature 1: Consumables== | ==Feature 1: Consumables== | ||
<!-- Instructions: "Consumables" is a term that biotech professionals and scientists use to describe non-machine supplies such as liquid items like reagents (PCR mix, primer solution, SYBR Green solution, buffer, etc.), plastic tubes, glass slides, etc. Many kits that are on the market include a few *very important* consumables (you can't include everything or else the box would be too large). What does *very important* mean? For instance, if your product includes a re-designed PCR machine with a different heating block that will not fit standard tubes, you may want to include tubes that are manufactured by your company. Summarize which consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | <!-- Instructions: "Consumables" is a term that biotech professionals and scientists use to describe non-machine supplies such as liquid items like reagents (PCR mix, primer solution, SYBR Green solution, buffer, etc.), plastic tubes, glass slides, etc. Many kits that are on the market include a few *very important* consumables (you can't include everything or else the box would be too large). What does *very important* mean? For instance, if your product includes a re-designed PCR machine with a different heating block that will not fit standard tubes, you may want to include tubes that are manufactured by your company. Summarize which consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | ||
Consumables: | |||
Glass slides that are 3/4 inch by 3 inches | |||
PCR mix | |||
Primer solution | |||
SYBR Green Solution | |||
Buffer solution | |||
Standard plastic tubes | |||
<!-- OPTIONAL: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | <!-- OPTIONAL: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> |
Revision as of 21:48, 24 November 2015
BME 100 Fall 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY - "Nappy Happy"
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System Seventeen teams of six students each diagnosed 34 patients total. Each team diagnosed two patients. There were multiple aspects to the lab that eliminated the error. The first thing was there was three replicates for each patient instead of just one. Then we also created a positive and negative control for the PCR, which we used water and the positive PCR, and water with negative PCR. The imageJ calibration controls were created by making six different concentrations of Calf Thymus DNA and relating those to the average amount of pixels of the drops. We took a total of 18 pictures, three pictures per concentration, for the calibrations. Next we took 24 pictures, three pictures of each the positive and negative controls.Then three pictures of each replicate of the patients. Our class data resulted with 34 total final results, with 2 No Tests, 2 Inconclusive tests,13 positive tests and 19 negative tests. Our group had two conclusive patients, one being positive and one being negative. We encountered a couple of problems throughout this process. When we were making the mixtures of PCR we were lacking some reagents, which made it difficult to replicate data for accuracy. We also encountered a problem figuring out how to do the final calculations, once we figured out what number to use we achieved our final data. What Bayes Statistics Imply about This Diagnostic Approach The results for calculation 1 is about 75% reliable that an individual receives a positive test result given a positive PCR reaction. The result for calculation 2 is close to 100% reliable that an individual receives a negative test result given a negative PCR reaction. The result for calculation 3 has a very low reliability that an individual will develop the disease when they get a positive test result. The result for calculation 4 has close to 100% reliability that an individual will not develop the disease when they get a negative test result. Three possible sources of error: 1) Lack of settings on the error so the image wasn't as clear as it could've possibly been 2) The distance of the camera to the drop could have varied throughout each trial 3) Lack of reagents given to us to create the drops Intro to Computer-Aided DesignTinkerCAD Our Design
Feature 1: ConsumablesConsumables: Glass slides that are 3/4 inch by 3 inches PCR mix Primer solution SYBR Green Solution Buffer solution Standard plastic tubes
Feature 2: Hardware - PCR Machine & Fluorimeter |