BME100 f2015:Group4 1030amL5

BME 100 Fall 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Gabrielle Mills Role(s) Name: Christa Deckman Role(s) Name: Neil Rastogi Role(s) Name: Ngan Nguyen Role(s) Name: Evan Higgs Role(s) Name: Dylan Bunch Role(s) LAB 5 WRITE-UP PCR Reaction Report Our pipetting of the samples was successful for the setup of this laboratory procedure. The appropriate amounts of DNA sample liquids and PCR reaction mix were added to the tubes with no observable error. The tubes were labeled as we planned and we successfully transferred the liquids between the tubes without contamination.The pre-lab provided appropriate information over how to complete a PCR lab. Through the interactive activities, we were able to familiarize ourselves with the important and significant procedure. In addition, the reading provided informative background descriptions and definitions that helped in the progression and completion of the laboratory procedure. The first and second stops on the pipettor were easy to distinguish after the first few pipetting steps. When you hit the first stop, it is quite hard to go past it accidentally. Therefore, when you purposefully expel the sample into the PCR tube, you can perceive when it is fully expelled at the second stop.The final reactions had the same amount of liquid as the original components combined. Precision was used in transferring the components into the reaction, and therefore, there was the exact same amount of liquid.There was no component liquids left in the tubes of DNA and the PCR reaction mix. Since precision was used in removing these liquids, there was no liquid left in these tubes and all was added into the PCR tubes.We used our original labeling scheme from Part A for the procedure in Part C. We saw no need to change it, given that it was unique to our group seeing as we labeled our PCR tubes with “G4.” Fluorimeter Procedure Smart Phone Camera Settings Type of Smartphone: Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Camera set-up Distance between the smart phone cradle and drop = Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HERE Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :